Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands. [In: Methods Related to DNA Sequence Recognition]
Hampshire, Andrew J., Rusling, David A., Broughton-Head, Victoria J. and Fox, Keith R. (2007) Footprinting: A method for determining the sequence selectivity, affinity and kinetics of DNA-binding ligands. [In: Methods Related to DNA Sequence Recognition]. Methods, 42, (2), 128-140. (doi:10.1016/j.ymeth.2007.01.002).
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Description/Abstract
Footprinting is a simple method for assessing the sequence selectivity of DNA-binding ligands. The method is based on the ability of the ligand to protect DNA from cleavage at its binding site. This review describes the use of DNase I and hydroxyl radicals, the most commonly used footprinting probes, in footprinting experiments. The success of a footprinting experiment depends on using an appropriate DNA substrate and we describe how these can best be chosen or designed. Although footprinting was originally developed for assessing a ligand’s sequence selectivity, it can also be employed to estimate the binding strength (quantitative footprinting) and to assess the association and dissociation rate constants for slow binding reactions.
| Item Type: | Article |
|---|---|
| ISSNs: | 1046-2023 (print) |
| Related URLs: | |
| Keywords: | footprinting, DNase I, hydroxyl radicals, sequence selectivity, dna binding |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56060 |
| Date Deposited: | 08 Aug 2008 |
| Last Modified: | 02 Mar 2012 13:54 |
| Contributors: | Hampshire, Andrew J. (Author) Rusling, David A. (Author) Broughton-Head, Victoria J. (Author) Fox, Keith R. (Author) |
| Date: | 1 June 2007 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56060 |
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