Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc

Lapham, A.S., Phillips, E.S. and Barton, C.H. (2004) Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc. Biochemical Society Transactions, 32, (6), 1084-1086.


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Slc11a1/Nramp1 (solute carrier family 11 member a1/murine natural resistance-associated macrophage protein 1 gene) encodes a divalent cation transporter that resides within lysosomes/late endosomes of macrophages. Nramp1 modulates the cellular distribution of divalent cations in response to cell activation by intracellular pathogens. Nramp1 expression is repressed and activated by the proto-oncogene c-Myc and Miz-1 (c-Myc-interacting zinc finger protein 1) respectively. Here we demonstrate, using a c-Myc mutant (V394D, Val394Asp) that is incapable of binding Miz-1, that c-Myc repression of Nramp1 transcription is dependent on its interaction with Miz-1. An oligo pull-down assay demonstrates specific binding of recombinant Miz-1 to the Nramp1 Miz-1-binding site or initiator element(s), and Miz-1-dependent c-Myc recruitment.

Item Type: Article
ISSNs: 0300-5127 (print)
Related URLs:
Subjects: Q Science > Q Science (General)
R Medicine > R Medicine (General)
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 56294
Accepted Date and Publication Date:
1 December 2004Published
Date Deposited: 06 Aug 2008
Last Modified: 31 Mar 2016 12:36

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