Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc
Lapham, A.S., Phillips, E.S. and Barton, C.H. (2004) Transcriptional control of Nramp 1: a paradigm for the repressive action of c-Myc. Biochemical Society Transactions, 32, (6), 1084-1086.
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Description/Abstract
Slc11a1/Nramp1 (solute carrier family 11 member a1/murine natural resistance-associated macrophage protein 1 gene) encodes a divalent cation transporter that resides within lysosomes/late endosomes of macrophages. Nramp1 modulates the cellular distribution of divalent cations in response to cell activation by intracellular pathogens. Nramp1 expression is repressed and activated by the proto-oncogene c-Myc and Miz-1 (c-Myc-interacting zinc finger protein 1) respectively. Here we demonstrate, using a c-Myc mutant (V394D, Val394Asp) that is incapable of binding Miz-1, that c-Myc repression of Nramp1 transcription is dependent on its interaction with Miz-1. An oligo pull-down assay demonstrates specific binding of recombinant Miz-1 to the Nramp1 Miz-1-binding site or initiator element(s), and Miz-1-dependent c-Myc recruitment.
| Item Type: | Article |
|---|---|
| ISSNs: | 0300-5127 (print) |
| Related URLs: | |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56294 |
| Date Deposited: | 06 Aug 2008 |
| Last Modified: | 11 Jun 2013 01:43 |
| Contributors: | Lapham, A.S. (Author) Phillips, E.S. (Author) Barton, C.H. (Author) |
| Date: | 1 December 2004 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56294 |
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