Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins
Long, A.R., Yang, M.Y., Kaiser, K. and Shepherd, D. (1998) Isolation and characterisation of smallminded, a Drosophila gene encoding a new member of the Cdc48p/VCP subfamily of AAA proteins. Gene, 208, (2), 191-199. (doi:10.1016/S0378-1119(97)00652-5).
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Description/Abstract
Smallminded (smid) encodes a new member of the cdc48p/VCP subfamily of AAA proteins in Drosophila. The gene was isolated by plasmid rescue from a GAL4 enhancer trap line which shows reporter gene expression in neuroblasts, imaginal disks and a subset of sensory neurons. Larvae homozygous for the insert arrest development as second instar larvae and die without pupating. The most obvious defect in these larvae is a significantly reduced CNS, hence the naming of the gene as smallminded. The deduced amino acid sequence of smid contains a tandem duplication of the AAA nucleotide binding domain characteristic of the cdc48p/VCP subfamily. Overall, smid shares 33% identical residues with its closest relative, yeast L0919-chrXII and 26–29% with other members of the cdc48p/VCP subfamily. The most highly conserved regions of the predicted protein structure are found in and around the nucleotide binding domains. The gene is expressed at all developmental stages.
| Item Type: | Article |
|---|---|
| ISSNs: | 0378-1119 (print) |
| Related URLs: | |
| Keywords: | cell cycle, enhancer-trap, Gal4, homotypic membrane fusion, neuroblast |
| Subjects: | R Medicine > R Medicine (General) Q Science > QR Microbiology > QR355 Virology Q Science > QR Microbiology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56299 |
| Date Deposited: | 21 Aug 2008 |
| Last Modified: | 01 Jun 2011 07:09 |
| Contributors: | Long, A.R. (Author) Yang, M.Y. (Author) Kaiser, K. (Author) Shepherd, D. (Author) |
| Date: | 22 February 1998 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56299 |
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