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Powdery mildew infection of wheat leaves changes host solute transport and invertase activity

Powdery mildew infection of wheat leaves changes host solute transport and invertase activity
Powdery mildew infection of wheat leaves changes host solute transport and invertase activity
The effect of powdery mildew (Blumeria graminis) infection of wheat leaves on solute transport and invertase activity of the host tissue has been examined. Sugars (glucose, sucrose, maltose) and amino acids (glutamine, histidine) were taken up by leaf pieces, and radioactivity was transferred to the fungal mycelium. Infection had a marked effect on sugar uptake, particularly for glucose which was taken up into infected tissue at considerably higher rates than into uninfected tissues. In contrast, amino acid uptake rates into infected tissues were lower when compared with those into uninfected tissue. The increase in glucose uptake could be correlated with a change in sugar transporter gene expression as a wheat homologue of the monosaccharide carrier AtSTP4 was shown to increase in infected tissue. Efflux analysis showed a higher leakage of preloaded glucose from infected leaves in comparison with uninfected tissue and transfer to the mycelium was greater for glucose than for the other solutes measured. All types of invertase, measured enzymatically, showed an increase in infected tissue, with the highest proportional increase observed for cell-wall invertase. A partial-length complementary DNA, TaINV2, was isolated for a putative cell-wall invertase; expression studies indicated that levels for this or related sequences increased substantially 3 days after infection.
0031-9317
787-795
Sutton, P.N.
1313a783-ed1c-4e20-9532-c557ec11b963
Gilbert, M.J.
ffd89802-d961-400d-a0cb-8e8e62386ba1
Williams, L.E.
79ee1856-3732-492b-8ac5-239749c85d9e
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4
Sutton, P.N.
1313a783-ed1c-4e20-9532-c557ec11b963
Gilbert, M.J.
ffd89802-d961-400d-a0cb-8e8e62386ba1
Williams, L.E.
79ee1856-3732-492b-8ac5-239749c85d9e
Hall, J.L.
24cd62e9-4050-4514-9446-5a03949007f4

Sutton, P.N., Gilbert, M.J., Williams, L.E. and Hall, J.L. (2007) Powdery mildew infection of wheat leaves changes host solute transport and invertase activity. Physiologia Plantarum, 129 (4), 787-795. (doi:10.1111/j.1399-3054.2007.00863.x).

Record type: Article

Abstract

The effect of powdery mildew (Blumeria graminis) infection of wheat leaves on solute transport and invertase activity of the host tissue has been examined. Sugars (glucose, sucrose, maltose) and amino acids (glutamine, histidine) were taken up by leaf pieces, and radioactivity was transferred to the fungal mycelium. Infection had a marked effect on sugar uptake, particularly for glucose which was taken up into infected tissue at considerably higher rates than into uninfected tissues. In contrast, amino acid uptake rates into infected tissues were lower when compared with those into uninfected tissue. The increase in glucose uptake could be correlated with a change in sugar transporter gene expression as a wheat homologue of the monosaccharide carrier AtSTP4 was shown to increase in infected tissue. Efflux analysis showed a higher leakage of preloaded glucose from infected leaves in comparison with uninfected tissue and transfer to the mycelium was greater for glucose than for the other solutes measured. All types of invertase, measured enzymatically, showed an increase in infected tissue, with the highest proportional increase observed for cell-wall invertase. A partial-length complementary DNA, TaINV2, was isolated for a putative cell-wall invertase; expression studies indicated that levels for this or related sequences increased substantially 3 days after infection.

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Published date: 1 April 2007

Identifiers

Local EPrints ID: 56426
URI: http://eprints.soton.ac.uk/id/eprint/56426
ISSN: 0031-9317
PURE UUID: 8e509231-9ee8-47c1-aa24-8feb01887ba5

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Date deposited: 08 Aug 2008
Last modified: 15 Mar 2024 11:01

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Contributors

Author: P.N. Sutton
Author: M.J. Gilbert
Author: L.E. Williams
Author: J.L. Hall

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