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Sequence effects of single base loops in intramolecular quadruplex DNA

Sequence effects of single base loops in intramolecular quadruplex DNA
Sequence effects of single base loops in intramolecular quadruplex DNA
We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1?,2?-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.
dna quadruplex, dna structure
0014-5793
1657-1660
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Rachwal, Phillip A.
8bd3be31-babc-456b-840e-6447b57c6999
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f

Rachwal, Phillip A., Brown, Tom and Fox, Keith R. (2007) Sequence effects of single base loops in intramolecular quadruplex DNA. FEBS Letters, 581 (8), 1657-1660. (doi:10.1016/j.febslet.2007.03.040).

Record type: Article

Abstract

We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1?,2?-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.

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More information

Submitted date: 7 February 2007
Published date: 17 April 2007
Keywords: dna quadruplex, dna structure

Identifiers

Local EPrints ID: 56440
URI: http://eprints.soton.ac.uk/id/eprint/56440
ISSN: 0014-5793
PURE UUID: 22e29e91-3956-4cee-82ec-190f62fa5e34
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 07 Aug 2008
Last modified: 16 Mar 2024 02:36

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Contributors

Author: Phillip A. Rachwal
Author: Tom Brown
Author: Keith R. Fox ORCID iD

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