Sequence effects of single base loops in intramolecular quadruplex DNA


Rachwal, Phillip A., Brown, Tom and Fox, Keith R. (2007) Sequence effects of single base loops in intramolecular quadruplex DNA. FEBS Letters, 581, (8), 1657-1660. (doi:10.1016/j.febslet.2007.03.040).

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Description/Abstract

We have examined the properties of intramolecular G-quadruplexes in which the G3 tracts are separated by single base loops. The most stable complex contained 1′,2′-dideoxyribose in all three loops, while loops containing T and C were slightly less stable (by about 2°C). Quadruplexes containing loops with single A residues were less stable by 8°C for each T to A substitution. These folded sequences display similar CD spectra, which are consistent with the formation of parallel stranded complexes with double-chain reversal loops. These results demonstrate that loop sequence, and not just length, affects quadruplex stability.

Item Type: Article
ISSNs: 0014-5793 (print)
Related URLs:
Keywords: dna quadruplex, dna structure
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Chemistry
ePrint ID: 56440
Date Deposited: 07 Aug 2008
Last Modified: 27 Mar 2014 18:39
Contact Email Address: k.r.fox@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/56440

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