In vivo expression of cyclooxygenase-2 in rat brain following intraparenchymal injection of bacterial endotoxin and inflammatory cytokines
In vivo expression of cyclooxygenase-2 in rat brain following intraparenchymal injection of bacterial endotoxin and inflammatory cytokines
To clarify the role played by prostaglandins in acute brain inflammation we studied the expression of the key enzyme in their formation, cyclooxygenase-2 (COX-2), following microinjection of bacterial endotoxin (LPS), interleukin-1 (3 (IL-I[beta]), tumor necrosis factor-a (TNF-[alpha]), and interferon--[gamma] (IFN--[gamma]), in the rat dorsal hippocampus. In spite of the extensive astrocyte and microglial reaction, at 24 hours after LPS injection COX-2 immunoreactivity (COX-2-ir) was exclusively associated with infiltrating neutrophils and with perivascular cells of blood vessels in the area surrounding the injection site. Microinjection of IFN-7 did not alter COX-2-ir, whereas TNF-[alpha] or IL-I[beta] injection induced a moderate COX-2-ir in the perivascular cells of a few blood vessels close to the injection site, and in very few of the infiltrating neutrophils. When ILI[beta], but not TNF-[alpha] or INF--[gamma], was injected in combination with LPS, a strong COX-2-ir was associated with the perivascular cells of most blood vessels in the injected hemisphere and of several of those in the uninjected hemisphere. In addition, COX- 2-ir was detected in neutrophils and in several parenchymal cells surrounding the injection site. The parenchymal and perivascular COX-2-positive cells showed a microglia/macrophage-like morphology, as compared with the GSI-B4 isolectin and ED-1 staining, specific for macrophages. Since the constitutive neuronal COX-2 was not affected by any of the conditions studied, the macrophage-like cells found in the perivascular region and in the parenchyma may represent the main source of prostaglandins during focal inflammatory responses in the brain.
1184-1191
Minghetti, L.
e5837f6e-d84f-4f4a-af54-fdf9c664e80b
Walsh, D.T.
872ce45b-409f-4ebf-838f-e011124e195a
Levi, G.
e1b01aa1-fe92-4302-8cb4-20bc9f55b483
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
November 1999
Minghetti, L.
e5837f6e-d84f-4f4a-af54-fdf9c664e80b
Walsh, D.T.
872ce45b-409f-4ebf-838f-e011124e195a
Levi, G.
e1b01aa1-fe92-4302-8cb4-20bc9f55b483
Perry, V.H.
8f29d36a-8e1f-4082-8700-09483bbaeae4
Minghetti, L., Walsh, D.T., Levi, G. and Perry, V.H.
(1999)
In vivo expression of cyclooxygenase-2 in rat brain following intraparenchymal injection of bacterial endotoxin and inflammatory cytokines.
Journal of Neuropathology & Experimental Neurology, 58 (11), .
Abstract
To clarify the role played by prostaglandins in acute brain inflammation we studied the expression of the key enzyme in their formation, cyclooxygenase-2 (COX-2), following microinjection of bacterial endotoxin (LPS), interleukin-1 (3 (IL-I[beta]), tumor necrosis factor-a (TNF-[alpha]), and interferon--[gamma] (IFN--[gamma]), in the rat dorsal hippocampus. In spite of the extensive astrocyte and microglial reaction, at 24 hours after LPS injection COX-2 immunoreactivity (COX-2-ir) was exclusively associated with infiltrating neutrophils and with perivascular cells of blood vessels in the area surrounding the injection site. Microinjection of IFN-7 did not alter COX-2-ir, whereas TNF-[alpha] or IL-I[beta] injection induced a moderate COX-2-ir in the perivascular cells of a few blood vessels close to the injection site, and in very few of the infiltrating neutrophils. When ILI[beta], but not TNF-[alpha] or INF--[gamma], was injected in combination with LPS, a strong COX-2-ir was associated with the perivascular cells of most blood vessels in the injected hemisphere and of several of those in the uninjected hemisphere. In addition, COX- 2-ir was detected in neutrophils and in several parenchymal cells surrounding the injection site. The parenchymal and perivascular COX-2-positive cells showed a microglia/macrophage-like morphology, as compared with the GSI-B4 isolectin and ED-1 staining, specific for macrophages. Since the constitutive neuronal COX-2 was not affected by any of the conditions studied, the macrophage-like cells found in the perivascular region and in the parenchyma may represent the main source of prostaglandins during focal inflammatory responses in the brain.
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Published date: November 1999
Organisations:
Biological Sciences
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Local EPrints ID: 56454
URI: http://eprints.soton.ac.uk/id/eprint/56454
ISSN: 0022-3069
PURE UUID: 3a6cd6b6-b1e1-48da-9737-05393b062867
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Date deposited: 21 Aug 2008
Last modified: 08 Jan 2022 04:01
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Author:
L. Minghetti
Author:
D.T. Walsh
Author:
G. Levi
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