The University of Southampton
University of Southampton Institutional Repository

Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain

Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain
Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain
Pectins are a family of complex cell-wall polysaccharides, the biosynthesis of which remains poorly understood. We identified dwarf mutants with reduced cell adhesion at a novel locus, QUASIMODO2 (QUA2). qua2-1 showed a 50% reduction in homogalacturonan (HG) content compared with the wild type, without affecting other cell-wall polysaccharides. The remaining HG in qua2-1 showed an unaltered degree of methylesterification. Positional cloning and GFP fusions showed that QUA2, consistent with a role in HG synthesis, encodes a Golgi-localized protein. In contrast to QUA1, another Golgi-localized protein required for HG-synthesis, QUA2 does not show sequence similarity to glycosyltransferases, but instead contains a putative methyltransferase (MT) domain. The Arabidopsis genome encodes 29 QUA2-related proteins. Interestingly, the transcript profiles of QUA1 and QUA2 are correlated and other pairs of QUA1 and QUA2 homologues with correlated transcript profiles can be identified. Together, the results lead to the hypothesis that QUA2 is a pectin MT, and that polymerization and methylation of homogalacturonan are interdependent reactions.
0960-7412
605-614
Mouille, G.
32979dbf-1815-42b3-8c01-9d6b24350e3c
Ralet, M.C.
1c986cfa-68f0-45ed-ad1a-d64ced083941
Cavelier, C.
ac4ffeb5-440d-4380-be0a-daaa24c197df
Eland, C.
312fe9e8-05f3-45da-bc5f-d3950206afd5
Effroy, D.
0ebbce01-5486-4fa1-8393-e54bd5e4b1eb
Hematy, K.
b3014d4b-8caf-4b2d-ae6c-39a9ec029d5b
McCartney, L.
2f958191-d0a9-440b-b632-e9c702801f53
Truong, H.N.
bc27631d-367a-4e06-867e-84915d9c052e
Gaudon, V.
543af97f-25d3-4bf6-8bfe-c3086a22e43b
Thibault, J.F.
31e46786-5d2b-4ade-b296-54d2ceaef1c8
Marchant, A.
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Hofte, H.
fa7d375a-2457-466e-8fea-98f7a31ca7f6
Mouille, G.
32979dbf-1815-42b3-8c01-9d6b24350e3c
Ralet, M.C.
1c986cfa-68f0-45ed-ad1a-d64ced083941
Cavelier, C.
ac4ffeb5-440d-4380-be0a-daaa24c197df
Eland, C.
312fe9e8-05f3-45da-bc5f-d3950206afd5
Effroy, D.
0ebbce01-5486-4fa1-8393-e54bd5e4b1eb
Hematy, K.
b3014d4b-8caf-4b2d-ae6c-39a9ec029d5b
McCartney, L.
2f958191-d0a9-440b-b632-e9c702801f53
Truong, H.N.
bc27631d-367a-4e06-867e-84915d9c052e
Gaudon, V.
543af97f-25d3-4bf6-8bfe-c3086a22e43b
Thibault, J.F.
31e46786-5d2b-4ade-b296-54d2ceaef1c8
Marchant, A.
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Hofte, H.
fa7d375a-2457-466e-8fea-98f7a31ca7f6

Mouille, G., Ralet, M.C., Cavelier, C., Eland, C., Effroy, D., Hematy, K., McCartney, L., Truong, H.N., Gaudon, V., Thibault, J.F., Marchant, A. and Hofte, H. (2007) Homogalacturonan synthesis in Arabidopsis thaliana requires a Golgi-localized protein with a putative methyltransferase domain. The Plant Journal, 50 (4), 605-614. (doi:10.1111/j.1365-313X.2007.03086.x).

Record type: Article

Abstract

Pectins are a family of complex cell-wall polysaccharides, the biosynthesis of which remains poorly understood. We identified dwarf mutants with reduced cell adhesion at a novel locus, QUASIMODO2 (QUA2). qua2-1 showed a 50% reduction in homogalacturonan (HG) content compared with the wild type, without affecting other cell-wall polysaccharides. The remaining HG in qua2-1 showed an unaltered degree of methylesterification. Positional cloning and GFP fusions showed that QUA2, consistent with a role in HG synthesis, encodes a Golgi-localized protein. In contrast to QUA1, another Golgi-localized protein required for HG-synthesis, QUA2 does not show sequence similarity to glycosyltransferases, but instead contains a putative methyltransferase (MT) domain. The Arabidopsis genome encodes 29 QUA2-related proteins. Interestingly, the transcript profiles of QUA1 and QUA2 are correlated and other pairs of QUA1 and QUA2 homologues with correlated transcript profiles can be identified. Together, the results lead to the hypothesis that QUA2 is a pectin MT, and that polymerization and methylation of homogalacturonan are interdependent reactions.

This record has no associated files available for download.

More information

Submitted date: 2 November 2006
Published date: 1 May 2007

Identifiers

Local EPrints ID: 56484
URI: http://eprints.soton.ac.uk/id/eprint/56484
ISSN: 0960-7412
PURE UUID: ff5dabf9-0ffe-4f66-8cfb-4f584333efc9

Catalogue record

Date deposited: 08 Aug 2008
Last modified: 15 Mar 2024 11:01

Export record

Altmetrics

Contributors

Author: G. Mouille
Author: M.C. Ralet
Author: C. Cavelier
Author: C. Eland
Author: D. Effroy
Author: K. Hematy
Author: L. McCartney
Author: H.N. Truong
Author: V. Gaudon
Author: J.F. Thibault
Author: A. Marchant
Author: H. Hofte

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×