Absence of detectable IL-1 beta production in murine prion disease: A model of chronic neurodegeneration
Walsh, D.T., Betmouni, S. and Perry, V.H. (2001) Absence of detectable IL-1 beta production in murine prion disease: A model of chronic neurodegeneration. Journal of Neuropathology & Experimental Neurology, 60, (2), 173-182.
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Description/Abstract
Murine prion disease is accompanied by a modified inflammatory response characterized by early but prolonged microglial activation and T-lymphocyte recruitment. In this model, we look at the profile of cytokine production, particularly IL-1[beta]. Mice inoculated with prion-infected brain homogenate show typical signs of prion disease. We were unable to detect any IL-1[beta] using immunohistochemistry, with various fixation protocols, or ELISA between 8 and 24 wk post-inoculation. Also, there was no increase in mRNA for IL-1[beta], IL-6, IFN[gamma], and iNOS as measured by quantitative RT-PCR. Using the same procedures and examining tissues at the same time, IL-1[beta] immunostaining was detected in infiltrating inflammatory cells in mouse brains injected with LPS or in a delayed-type hypersensitivity response in the brain. Soluble IL-1[beta] was also increased, as measured by ELISA, and there was an increase in mRNA species for IL-1[beta], IL-6, TNF[alpha] but not IFN[gamma] or iNOS in these brains. These data reveal that chronic neurodegeneration seen in prion disease does not induce production of a range of proinflammatory mediators despite showing marked microglial activation and raise the question as to whether IL-1[beta] would exacerbate the neurodegeneration as it does in acute neurodegeneration following head injury and stroke.
| Item Type: | Article |
|---|---|
| ISSNs: | 0022-3069 (print) |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56490 |
| Date Deposited: | 08 Aug 2008 |
| Last Modified: | 12 May 2013 01:15 |
| Contributors: | Walsh, D.T. (Author) Betmouni, S. (Author) Perry, V.H. (Author) |
| Date: | February 2001 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56490 |
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