Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor


Badasso, M.O., Dhanaraj, V., Wood, S.P., Cooper, J.B. and Blundell, T.L. (2004) Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor. Acta Crystallographica Section D, Biological Crystallography, 60, 770-772. (doi:10.1107/S0907444904003221).

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Description/Abstract

Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 {(2R,3S)-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate} for X-ray crystallography. Tetragonal complex crystals with space group P43212 were produced by the hanging-drop vapour-diffusion method and diffracted to 3.0 Å. The crystals exhibited unit-cell parameters a = b = 182.5, c = 99.1 Å and contained four molecules in the asymmetric unit. A 96% complete data set was collected at 298 K using Cu K X-rays from a rotating-anode generator. Solution of the crystal structure of Y75N mutant M. pusillus pepsin is under way by molecular replacement using the molecular coordinates of wild-type M. pusillus pepsin as a model.

Item Type: Article
ISSNs: 0907-4449 (print)
Related URLs:
Keywords: pepsins, aspartic proteinases, inhibitors,
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 56502
Date Deposited: 06 Aug 2008
Last Modified: 27 Mar 2014 18:39
URI: http://eprints.soton.ac.uk/id/eprint/56502

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