Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor
Badasso, M.O., Dhanaraj, V., Wood, S.P., Cooper, J.B. and Blundell, T.L. (2004) Crystallization and X-ray analysis of the Y75N mutant of Mucor pusillus pepsin complexed with inhibitor. Acta Crystallographica Section D, Biological Crystallography, 60, 770-772. (doi:10.1107/S0907444904003221).
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Description/Abstract
Y75N mutant Mucor pusillus pepsin has been overexpressed in yeast, purified and cocrystallized with the iodine-containing human renin inhibitor CP-113972 {(2R,3S)-isopropyl 3-[(L-prolyl-p-iodo-L-phenylalanyl-S-methyl-cysteinyl)amino-4]-cyclohexyl-2-hydroxybutanoate} for X-ray crystallography. Tetragonal complex crystals with space group P43212 were produced by the hanging-drop vapour-diffusion method and diffracted to 3.0 Å. The crystals exhibited unit-cell parameters a = b = 182.5, c = 99.1 Å and contained four molecules in the asymmetric unit. A 96% complete data set was collected at 298 K using Cu K X-rays from a rotating-anode generator. Solution of the crystal structure of Y75N mutant M. pusillus pepsin is under way by molecular replacement using the molecular coordinates of wild-type M. pusillus pepsin as a model.
| Item Type: | Article |
|---|---|
| ISSNs: | 0907-4449 (print) |
| Related URLs: | |
| Keywords: | pepsins, aspartic proteinases, inhibitors, |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56502 |
| Date Deposited: | 06 Aug 2008 |
| Last Modified: | 12 May 2013 01:15 |
| Contributors: | Badasso, M.O. (Author) Dhanaraj, V. (Author) Wood, S.P. (Author) Cooper, J.B. (Author) Blundell, T.L. (Author) |
| Date: | 1 April 2004 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56502 |
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