Switched-angle spinning applied to bicelles containing phospholipid-associated peptides
Zandomeneghi, G., Williamson, P.T.F., Hunkeler, A. and Meier, B.H. (2003) Switched-angle spinning applied to bicelles containing phospholipid-associated peptides. Journal of Biomolecular Nuclear Magnetic Resonance, 25, (2), 125-132. (doi:10.1023/A:1022244025351).
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In a model study, the proton NMR spectrum of the opioid pentapeptide leucine-enkephalin associated with bicelles is investigated. The spectral resolution for a static sample is limited due to the large number of anisotropic interactions, in particular strong proton–proton couplings, but resolution is greatly improved by magic-angle sample spinning. Here we present two-dimensional switched-angle spinning NMR experiments, which correlate the high-resolution spectrum of the membrane-bound peptide under magic-angle spinning with its anisotropic spectrum, leading to well-resolved spectra. The two-dimensional spectrum allows the exploitation of the high resolution of the isotropic spectrum, while retaining the structural information imparted by the anisotropic interactions in the static spectrum. Furthermore, switched-angle spinning techniques are demonstrated that allow one to record the proton spectrum of ordered bicellar phases as a function of the angle between the rotor axis and the magnetic field direction, thereby scaling the dipolar interactions by a predefined factor.
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||07 Aug 2008|
|Last Modified:||01 Jun 2011 04:54|
|Contributors:||Zandomeneghi, G. (Author)
Williamson, P.T.F. (Author)
Hunkeler, A. (Author)
Meier, B.H. (Author)
|Date:||1 February 2003|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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