Switched-angle spinning applied to bicelles containing phospholipid-associated peptides
Zandomeneghi, G., Williamson, P.T.F., Hunkeler, A. and Meier, B.H. (2003) Switched-angle spinning applied to bicelles containing phospholipid-associated peptides. Journal of Biomolecular Nuclear Magnetic Resonance, 25, (2), 125-132. (doi:10.1023/A:1022244025351).
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Description/Abstract
In a model study, the proton NMR spectrum of the opioid pentapeptide leucine-enkephalin associated with bicelles is investigated. The spectral resolution for a static sample is limited due to the large number of anisotropic interactions, in particular strong proton–proton couplings, but resolution is greatly improved by magic-angle sample spinning. Here we present two-dimensional switched-angle spinning NMR experiments, which correlate the high-resolution spectrum of the membrane-bound peptide under magic-angle spinning with its anisotropic spectrum, leading to well-resolved spectra. The two-dimensional spectrum allows the exploitation of the high resolution of the isotropic spectrum, while retaining the structural information imparted by the anisotropic interactions in the static spectrum. Furthermore, switched-angle spinning techniques are demonstrated that allow one to record the proton spectrum of ordered bicellar phases as a function of the angle between the rotor axis and the magnetic field direction, thereby scaling the dipolar interactions by a predefined factor.
| Item Type: | Article |
|---|---|
| ISSNs: | 0925-2738 (print) |
| Related URLs: | |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56545 |
| Date Deposited: | 07 Aug 2008 |
| Last Modified: | 01 Jun 2011 04:54 |
| Contributors: | Zandomeneghi, G. (Author) Williamson, P.T.F. (Author) Hunkeler, A. (Author) Meier, B.H. (Author) |
| Date: | 1 February 2003 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56545 |
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