Crystallization and preliminary X-ray analysis of the controller protein C.Ahdl from Aeromonas hydrophilia
McGeehan, J.E., Streeter, S., Cooper, J.B., Mohammed, F., Fox, G.C. and Kneale, G.G. (2004) Crystallization and preliminary X-ray analysis of the controller protein C.Ahdl from Aeromonas hydrophilia. Acta Crystallographica Section D: Biological Crystallography, 60, (2), 323-325. (doi:10.1107/S0907444903026143).
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Description/Abstract
Single crystals of purified homodimeric controller protein from Aeromonas hydrophilia (C.AhdI) have been grown under several different conditions using vapour diffusion. X-ray diffraction data have been collected using synchrotron radiation from crystals of both the native and a selenomethionine (SeMet) derivative of the protein. The native crystal form belongs to space group P21 and data were collected to a resolution of 2.2 Å. Two crystal forms of the SeMet protein have been obtained and were found to belong to space groups P1 and P21; data have been recorded to 2.0 and 1.7 Å resolution, respectively, for the two crystal forms. Three-wavelength MAD data were collected to 1.7 Å for the SeMet derivative crystal, which is isomorphous with the native P21 crystal.
| Item Type: | Article |
|---|---|
| ISSNs: | 0907-4449 (print) |
| Related URLs: | |
| Keywords: | aeromonas hydrophilia, dna-binding proteins, dna-modification methylases |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56682 |
| Date Deposited: | 06 Aug 2008 |
| Last Modified: | 12 May 2013 01:17 |
| Contributors: | McGeehan, J.E. (Author) Streeter, S. (Author) Cooper, J.B. (Author) Mohammed, F. (Author) Fox, G.C. (Author) Kneale, G.G. (Author) |
| Date: | 1 February 2004 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56682 |
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