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Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods

Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods
Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods
mGluRs (metabotropic glutamate receptors) are G-protein-coupled receptors that modulate synaptic transmission. The eight mammalian mGluRs form three groups based on sequence and functional similarities: group I (1 and 5), group II (2 and 3) and group III (4, 6–8) mGluRs. In the present study, we used a Y2H (yeast two hybrid) screen to identify proteins that interact with the C-terminal intracellular tail of mGluR3. Prominent among the candidate receptor interacting proteins was calmodulin, a Ca2+ sensor known to bind identifiable sequences in group I and III mGluRs. The Y2H method was used to investigate calmodulin binding to mGluRs but failed to confirm the documented interaction with group III mGluRs. Furthermore, subsequent biochemical analysis showed that calmodulin does not interact with group II mGluRs. This illustrates that certain Ca2+-dependent interactions are not recapitulated in yeast. Moreover, it highlights the necessity for supporting biochemical data to substantiate interactions identified with Y2H methods.
calmodulin, metabotropic glutamate receptor (mGluR), protein interaction, yeast two-hybrid (Y2H) screen
0300-5127
868-870
Lidwell, K.
7bc7781a-ab37-4fed-8cf7-4207b46a5866
Dillon, J.
f406e30a-3ad4-4a53-80db-6694bab5e3ed
Sihota, A.
ebdd3702-300c-4b38-84ac-f332e6b76fe0
Connor, V.
bc8fea0c-2bd2-48a3-8ca6-e747ffd6298d
Pilkington, B.
66ffd9b8-053f-4445-884f-08d7165e72af
Lidwell, K.
7bc7781a-ab37-4fed-8cf7-4207b46a5866
Dillon, J.
f406e30a-3ad4-4a53-80db-6694bab5e3ed
Sihota, A.
ebdd3702-300c-4b38-84ac-f332e6b76fe0
Connor, V.
bc8fea0c-2bd2-48a3-8ca6-e747ffd6298d
Pilkington, B.
66ffd9b8-053f-4445-884f-08d7165e72af

Lidwell, K., Dillon, J., Sihota, A., Connor, V. and Pilkington, B. (2004) Determining calmodulin binding to metabotropic glutamate receptors interaction methods with distinct protein-interaction methods. Biochemical Society Transactions, 32 (5), 868-870.

Record type: Article

Abstract

mGluRs (metabotropic glutamate receptors) are G-protein-coupled receptors that modulate synaptic transmission. The eight mammalian mGluRs form three groups based on sequence and functional similarities: group I (1 and 5), group II (2 and 3) and group III (4, 6–8) mGluRs. In the present study, we used a Y2H (yeast two hybrid) screen to identify proteins that interact with the C-terminal intracellular tail of mGluR3. Prominent among the candidate receptor interacting proteins was calmodulin, a Ca2+ sensor known to bind identifiable sequences in group I and III mGluRs. The Y2H method was used to investigate calmodulin binding to mGluRs but failed to confirm the documented interaction with group III mGluRs. Furthermore, subsequent biochemical analysis showed that calmodulin does not interact with group II mGluRs. This illustrates that certain Ca2+-dependent interactions are not recapitulated in yeast. Moreover, it highlights the necessity for supporting biochemical data to substantiate interactions identified with Y2H methods.

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More information

Published date: 1 November 2004
Keywords: calmodulin, metabotropic glutamate receptor (mGluR), protein interaction, yeast two-hybrid (Y2H) screen

Identifiers

Local EPrints ID: 56683
URI: http://eprints.soton.ac.uk/id/eprint/56683
ISSN: 0300-5127
PURE UUID: 88e4a00f-332d-439b-8868-3b711e641d49
ORCID for J. Dillon: ORCID iD orcid.org/0000-0003-3244-7483

Catalogue record

Date deposited: 06 Aug 2008
Last modified: 29 Mar 2022 01:38

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Contributors

Author: K. Lidwell
Author: J. Dillon ORCID iD
Author: A. Sihota
Author: V. Connor
Author: B. Pilkington

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