Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease


Sardy, M., Csikos, M., Geisen, C., Preisz, K., Kornsee, Z., Tomsits, E., Tox, U., Hunzelmann, N., Wieslander, J., Karpati, S., Paulsson, M. and Smyth, N. (2007) Tissue transglutaminase ELISA positivity in autoimmune disease independent of gluten-sensitive disease. Clinica Chimica Acta, 376, (1-2), 126-135. (doi:10.1016/j.cca.2006.08.006).

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Description/Abstract

Background
Our aim was to understand why some sera from patients with a broad spectrum of autoimmune diseases or non-autoimmune diseases involving enhanced apoptosis, cell lysis and/or putative secondary autoimmune processes show reactions in the tissue transglutaminase (TGc) ELISA used for diagnosis of gluten-sensitive disease.

Methods
Sera were compared from groups of patients with autoimmune diseases, diseases involving organ specific enhanced cell death, celiac disease or dermatitis herpetiformis, diseases of non-autoimmune origin, and a group without known disease. IgA antibodies against TGc were detected using human antigen (produced recombinantly in bacterial or human cells) in different systems (non-commercial ELISA with buffers of differing NaCl concentrations, and anti-TGc sandwich ELISA). Anti-gliadin and anti-endomysium antibodies were also determined.

Results
Many sera from patients with autoimmune disorders gave a positive signal in the human TGc ELISAs. The signal appeared related to minor impurities in the recombinant human TGc used and to raised serum IgA antibody levels rather than to the occurrence of TGc specific antibodies in these patients.

Conclusions
No association of anti-TGc Abs and autoimmune conditions independent of gluten-sensitive disease could be shown. Care should be taken to exclude copurification of chaperones, like heat shock protein 70, where preparing antigens for TGc ELISAs.

Keywords: Tissue transglutaminase; Celiac disease; Gluten-sensitive disease; Autoimmunity; ELISA

Item Type: Article
ISSNs: 0009-8981 (print)
Related URLs:
Keywords: tissue transglutaminase, celiac disease, gluten-sensitive disease, autoimmunity, ELISA
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 56733
Date Deposited: 08 Aug 2008
Last Modified: 27 Mar 2014 18:39
URI: http://eprints.soton.ac.uk/id/eprint/56733

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