Residues 1 to 80 of the N-terminal domain of the β subunit confer neuronal bungarotoxin sensitivity and agonist selectivity on neuronal nicotinic receptors
Wheeler, Susan V., Chad, John E. and Foreman, Richard (1993) Residues 1 to 80 of the N-terminal domain of the β subunit confer neuronal bungarotoxin sensitivity and agonist selectivity on neuronal nicotinic receptors. Federation of European Biochemical Societies (FEBS) Letters, 332, (1-2), 139-142. (doi:10.1016/0014-5793(93)80500-T).
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Standard two electrode voltage clamp techniques were used to investigate the response of neuronal nicotinic acetylcholine receptors, expressed in Xenopus oocytes, to various agonists and neuronal bungarotoxin (NBT). The β subunit is an important determinant of the receptor's pharmacological profile. Co-expression of α4 and β2 subunits produced a receptor that was relatively insensitive to cytisine and nicotine and inhibited by NBT, whilst the α4β4 combination produced a receptor that was highly sensitive to cytisine and nicotine but resistant to toxin. The first 80 amino acids of the N-terminal domain of the β subunit are implicated in these characteristics, since the combination of α4 with a hybrid β subunit comprising amino acids 1 → 80 of β2 and 81 → 416 of β4 became relatively insensitive to nicotine and cytisine and resistant to inhibition by neuronal bungarotoxin.
|Keywords:||nicotinic receptor, chimeric subunit, xenopus oocyte, neuronal bungarotoxin|
|Subjects:||Q Science > Q Science (General)
Q Science > QR Microbiology
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||22 Aug 2008|
|Last Modified:||02 Mar 2012 13:32|
|Contributors:||Wheeler, Susan V. (Author)
Chad, John E. (Author)
Foreman, Richard (Author)
|Date:||11 October 1993|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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