The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase
Botella, J.A., Baines, I.A., Williams, D.D., Goberdhan, D.C.I., Proud, C.G. and Wilson, C. (2001) The Drosophila cell shape regulator c-Jun N-terminal kinase also functions as a stress-activated protein kinase. Insect Biochemistry and Molecular Biology, 31, (9), 839-847. (doi:10.1016/S0965-1748(01)00029-7).
Full text not available from this repository.
Mammalian c-Jun N-terminal kinases (JNKs) are members of a group of stress-activated intracellular signalling molecules within the MAP kinase family. Molecular genetic analysis of a highly evolutionarily conserved Drosophila JNK homologue, DJNK, has demonstrated that this molecule plays an essential developmental role in cell shape regulation. However, it remains to be determined whether DJNK also responds to the broad range of cellular stresses and other stimuli that affect its mammalian counterpart. Here we demonstrate that c-Jun, a substrate for mammalian JNKs, is a specific substrate for DJNK and that an antiserum that cross-reacts with activated mammalian JNK at the conserved threonyl-prolyl-tyrosyl (TPY) motif within the activation loop also specifically recognises the activated form of DJNK. Using these two assays, we show that DJNK activity is stimulated in cultured cells by several treatments that activate mammalian JNKs, including addition of arsenite, vanadate and ceramide derivatives. It is therefore concluded that in addition to its essential developmental functions, DJNK plays an important role in stress responses that mirrors its mammalian counterpart.
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||08 Aug 2008|
|Last Modified:||12 May 2013 01:18|
|Contributors:||Botella, J.A. (Author)
Baines, I.A. (Author)
Williams, D.D. (Author)
Goberdhan, D.C.I. (Author)
Proud, C.G. (Author)
Wilson, C. (Author)
|Date:||1 July 2001|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
Actions (login required)