Increased perinuclear Ca2+ activity evoked by metabotropic glutamate receptor activation in rat hippocampal neurones
Phenna, S., Jane, S.D. and Chad, J.E. (1995) Increased perinuclear Ca2+ activity evoked by metabotropic glutamate receptor activation in rat hippocampal neurones. Journal of Physiology, 486, (Pt 1), 149-161.
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Description/Abstract
1. The effect of metabotropic glutamate receptor activation on intracellular Ca2+ activity (alpha Cai) of rat hippocampal pyramidal neurones in vitro was examined using ratiometric confocal laser scanning microscopy with the Ca(2+)-sensitive fluorescent probe indo-1 AM. 2. Metabotropic receptors were selectively activated with 1S,3R-1-aminocyclopentane-1,3-dicarboxylic acid (1S,3R-ACPD; 100 microM) in the presence of D-2-amino 5-phosphonovaleric acid (D-APV), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and CdCl2. Most pyramidal neurones (77/84) responded with an elevation in Ca2+ activity, maximal after 3-5 min. Fluorescence ratio responses were concentration dependent (EC50 approximately 10 microM) and were blocked by prior application of the antagonist (RS)-4-carboxy-3-hydroxyphenylglycine (RS-CHPG, 300 microM). 3. Responses to 1S,3R-ACPD (100 microM) also caused acidification of the neurones, from estimated control pH 7.2 to pH 6.6 (measured with the pH-sensitive dye SNAFL-calcein). The correction factor for indo-1 determination of Ca2+ was estimated to be x 1.4. 4. Elevations in alpha Cai were greater within the perinuclear region (> 1000 nM), than in the cytoplasm (approximately 200 nM). This region was devoid of staining by the endoplasmic reticulum staining dye 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)). 5. It is concluded that activation of metabotropic receptors in immature rat hippocampal pyramidal neurones leads to a large increase in perinuclear Ca2+ which would be well positioned to interact with the genome.
| Item Type: | Article |
|---|---|
| ISSNs: | 0022-3751 (print) |
| Related URLs: | |
| Subjects: | Q Science > Q Science (General) Q Science > QL Zoology Q Science > QR Microbiology |
| Divisions: | University Structure - Pre August 2011 > School of Biological Sciences |
| Item ID: | 56778 |
| Date Deposited: | 22 Aug 2008 |
| Last Modified: | 02 Mar 2012 13:54 |
| Contributors: | Phenna, S. (Author) Jane, S.D. (Author) Chad, J.E. (Author) |
| Date: | July 1995 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/56778 |
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