Fiber ternplating of poly(2-hydroxyethyl methacrylate) for neural tissue engineering
Flynn, Lauren, Dalton, Paul D. and Shoichet, Molly S. (2003) Fiber ternplating of poly(2-hydroxyethyl methacrylate) for neural tissue engineering. Biomaterials, 24, (23), 4265-4272. (doi:10.1016/S0142-9612(03)00334-X).
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We have developed a method to create longitudinally oriented channels within poly(2-hydroxyethyl methacrylate) (pHEMA) hydrogels for neural tissue engineering applications. Incorporated into an entubulation strategy, these scaffolds have the potential to enhance nerve regeneration after transection injuries of either the spinal cord or the peripheral nerve by increasing the available surface area and providing guidance to extending axons and invading cells. The fabrication process is straightforward and the resultant scaffolds are highly reproducible. Polycaprolactone (PCL) previous termfibersnext term were extruded and embedded in transparent, crosslinked pHEMA gels. Sonication of the pHEMA/PCL composite in acetone resulted in the complete dissolution of the PCL, leaving longitudinally oriented, previous termfibernext term-free channels in the pHEMA gel. Regulating the size and quantity of the PCL previous termfibersnext term allowed us to control the diameter and number of channels. Small and large channel scaffolds were fabricated and thoroughly characterized. The small channel scaffolds had 142±7 channels, with approximately 75% of the channels in the 100–200 μm size range. The large channel scaffolds had 37±1 channels, with approximately 77% of the channels in the 300–400 μm range. The equilibrium water content (EWC), porosity and compressive modulus were measured for each of the structures. Small and large channel scaffolds had, respectively, EWCs of 55.0±1.2% and 56.2±2.9%, porosities of 35±1% and 40±1% and compressive moduli of 191±7 and 182±4 kPa.
|Keywords:||pHEMA, polycaprolactone, fibers, spinal cord injury, regeneration, nerve guidance channel, scaffold, orientation, hydrogel|
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Divisions:||University Structure - Pre August 2011 > School of Biological Sciences
|Date Deposited:||07 Aug 2008|
|Last Modified:||01 Jun 2011 14:46|
|Contributors:||Flynn, Lauren (Author)
Dalton, Paul D. (Author)
Shoichet, Molly S. (Author)
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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