Effect of G-tract length on the topology and stability of intramolecular DNA quadruplexes

Rachwal, Philip A., Brown, Tom and Fox, Keith R. (2007) Effect of G-tract length on the topology and stability of intramolecular DNA quadruplexes. Biochemistry, 46, (11), 3036-3044. (doi:10.1021/bi062118j).


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Original Publication URL: http://dx.doi.org/10.1021/bi062118j


G-Rich sequences are known to form four-stranded structures that are based on stacks of G-quartets, and sequences with the potential to adopt these structures are common in eukaryotic genomes. However, there are few rules for predicting the relative stability of folded complexes that are adopted by sequences with different-length G-tracts or variable-length linkers between them. We have used thermal melting, circular dichroism, and gel electrophoresis to examine the topology and stability of intramolecular G-quadruplexes that are formed by sequences of the type d(GnT)4 and d(GnT2)4 (n = 3-7) in the presence of varying concentrations of sodium and potassium. In the presence of potassium or sodium, d(GnT)4 sequences form intramolecular parallel complexes with the following order of stability: n = 3 > n = 7 > n = 6 > n = 5 > n = 4. d(G3T)4 is anomalously stable. In contrast, the stability of d(GnT2)4 increases with the length of the G-tract (n = 7 > n = 6 > n = 5 > n = 4 > n = 3). The CD spectra for d(GnT)4 in the presence of potassium exhibit positive peaks around 260 nm, consistent with the formation of parallel topologies. These peaks are retained in sodium-containing buffers, but when n = 4, 5, or 6, CD maxima are observed around 290 nm, suggesting that these sequences [especially d(G5T)4] have some antiparallel characteristics. d(G3T2)4 adopts a parallel conformation in the presence of both sodium and potassium, while all the other d(GnT2)4 complexes exhibit predominantly antiparallel features. The properties of these complexes are also affected by the rate of annealing, and faster rates favor parallel complexes.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1021/bi062118j
ISSNs: 0006-2960 (print)
Related URLs:
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 56823
Accepted Date and Publication Date:
20 March 2007Published
11 October 2006Submitted
Date Deposited: 07 Aug 2008
Last Modified: 31 Mar 2016 12:37
URI: http://eprints.soton.ac.uk/id/eprint/56823

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