Fabrication and testing of a micro-fabricated PCR Device with an integrated fluorescence detector
Bahrami, M., Evans, A.G.R., Wilkinson, J.S., Husband, B., Sessions, N.P. and Melvin, T. (2005) Fabrication and testing of a micro-fabricated PCR Device with an integrated fluorescence detector. In, 16th MicroMechanics Europe Workshop (MME 2005), Gothenburg, Sweden, 04 - 06 Sep 2005.
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In this paper, we report the complete microfabrication of a polymerase chain reaction (PCR) device with both integrated temperature cycling and fluorescence detection elements. Also experimental data of temperature cycling and fluorescence detection system which includes an integrated photodiode covered by a thin film of cadmium sulphide as an optical filter are presented. Experiments indicate that temperature cycling is much faster than conventional PCR thermocycler also the composition of the photodiode and optical filter produces enough attenuation at excitation wavelength for fluorescence detection.
|Item Type:||Conference or Workshop Item (Paper)|
|Subjects:||Q Science > QC Physics
T Technology > TK Electrical engineering. Electronics Nuclear engineering
|Divisions:||University Structure - Pre August 2011 > Optoelectronics Research Centre
|Date Deposited:||11 Aug 2008|
|Last Modified:||30 May 2014 10:41|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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