Lectin binding sites on Euplotes mutabilis (Tuffrau, 1960) and the implications for food particle selection


Wilks, Sandra A. and Sleigh, Michael A. (2004) Lectin binding sites on Euplotes mutabilis (Tuffrau, 1960) and the implications for food particle selection. European Journal of Protistology, 40, (2), 153-162. (doi:10.1016/j.ejop.2004.01.004).

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Description/Abstract

Seven of the nine lectins tested bound to food vacuolar membranes and/or the cytostome of the filter-feeding ciliate Euplotes mutabilis. Strongest binding to the cytostome was found with concanavalin A, which labelled the cytostomes of more than 90% of cells within 10 s, and quickly labelled progressively more of the food vacuolar membrane material (80% of cells had labelled food vacuoles after 50 s exposure to the lectin). Wheat germ agglutinin specifically labelled the basal halves of a particular group of 8-9 frontal membranelles of every cell within 10 s, as well as labelling the cytostome area and food vacuoles of many cells. The specific areas of the cells which bind these lectins are involved in food capture. The frontal membranelles are involved in the interception of particles from the feeding current. The cytostome membrane bears binding sites to lectins of many types; these binding sites may be involved in the selective capture of food particles destined for ingestion, whilst unbound particles may be rejected.

Item Type: Article
ISSNs: 0932-4739 (print)
Related URLs:
Keywords: concanavalin A, Euplotes mutabilis, filter feeding ciliate, food selection, lectins, wheat germ agglutinin
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Biological Sciences
ePrint ID: 58178
Date Deposited: 12 Aug 2008
Last Modified: 27 Mar 2014 18:40
Contact Email Address: s.a.wilks@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/58178

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