Airway neutrophilia in COPD is not associated with increased neutrophil survival
Airway neutrophilia in COPD is not associated with increased neutrophil survival
Neutrophilic airway inflammation is a prominent feature of chronic obstructive pulmonary disease (COPD) and correlates with disease severity. The mechanisms that determine the extent of neutrophilia could involve increased influx or prolonged survival of neutrophils. We wanted to assess whether neutrophil pro-survival mechanisms are increased in the airways of subjects with COPD due to the presence of anti-apoptotic factors in the bronchial lining fluid.Induced sputum samples were collected from 20 subjects with stable COPD, 14 healthy smokers and 14 healthy controls. Quantification of apoptotic neutrophils was based on typical morphological cell changes. The anti-apoptotic, pro-survival activity in the sputum was studied by culturing peripheral blood neutrophils with the fluid phase of induced sputum. Apoptosis was assessed both by morphology and flow cytometry using Annexin-7AAD staining. COPD-patients and healthy smokers had a significantly higher percentage of sputum neutrophils than healthy controls (p<0.0001, p=0.01). However, there were no significant differences between the three subject groups in either the proportion of apoptotic neutrophils in sputum or the in vitro anti-apoptotic activity detected in the sputum fluid phase. Prolonged survival of neutrophils in sputum is not a feature of COPD and cannot explain the increased numbers of airway neutrophils in this disease.
1163-1169
Rytil, P.
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Plataki, M.
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Bucchieri, F.
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Uddin, M.
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Nong, G.
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Kinnula, V.L.
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Djukanovic, R.
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13 September 2006
Rytil, P.
b07f204c-81bc-4015-baa7-9d0cee84fc35
Plataki, M.
8ff4be5d-07e6-46eb-b346-23e9c2943859
Bucchieri, F.
830cf621-3e39-42ab-b0ea-7d5dc43bf032
Uddin, M.
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Nong, G.
c4d83202-2e1a-4501-9903-ddcdc9776f47
Kinnula, V.L.
c5258299-9380-45f2-9614-ce80e40d83f2
Djukanovic, R.
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Rytil, P., Plataki, M., Bucchieri, F., Uddin, M., Nong, G., Kinnula, V.L. and Djukanovic, R.
(2006)
Airway neutrophilia in COPD is not associated with increased neutrophil survival.
European Respiratory Journal, 28 (6), .
(doi:10.1183/09031936.00149005).
Abstract
Neutrophilic airway inflammation is a prominent feature of chronic obstructive pulmonary disease (COPD) and correlates with disease severity. The mechanisms that determine the extent of neutrophilia could involve increased influx or prolonged survival of neutrophils. We wanted to assess whether neutrophil pro-survival mechanisms are increased in the airways of subjects with COPD due to the presence of anti-apoptotic factors in the bronchial lining fluid.Induced sputum samples were collected from 20 subjects with stable COPD, 14 healthy smokers and 14 healthy controls. Quantification of apoptotic neutrophils was based on typical morphological cell changes. The anti-apoptotic, pro-survival activity in the sputum was studied by culturing peripheral blood neutrophils with the fluid phase of induced sputum. Apoptosis was assessed both by morphology and flow cytometry using Annexin-7AAD staining. COPD-patients and healthy smokers had a significantly higher percentage of sputum neutrophils than healthy controls (p<0.0001, p=0.01). However, there were no significant differences between the three subject groups in either the proportion of apoptotic neutrophils in sputum or the in vitro anti-apoptotic activity detected in the sputum fluid phase. Prolonged survival of neutrophils in sputum is not a feature of COPD and cannot explain the increased numbers of airway neutrophils in this disease.
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Published date: 13 September 2006
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Local EPrints ID: 59400
URI: http://eprints.soton.ac.uk/id/eprint/59400
ISSN: 0903-1936
PURE UUID: 8cd58f94-ac22-460e-baa7-22da9861fa3e
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Date deposited: 03 Sep 2008
Last modified: 16 Mar 2024 02:36
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Author:
P. Rytil
Author:
M. Plataki
Author:
F. Bucchieri
Author:
M. Uddin
Author:
G. Nong
Author:
V.L. Kinnula
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