Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR of X and Y chromosome markers for quality control of epidemiological DNA banks
Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR of X and Y chromosome markers for quality control of epidemiological DNA banks
Large DNA banks for human epidemiological studies have become an increasingly important research tool. The power of genotype-phenotype studies is dependent both on the quality of phenotyping and of genotyping and of correct linking of phenotypes to genotypes. Samples must be tracked through numerous steps between subject or patient and post-genotypic data. Only one phenotype, sex, has a perfect and binary correlation with genotype. In mixed sex studies, it may be advantageous for purposes of quality control to keep sexes mixed during the steps from acquisition to DNA bank, in order to be able to check later for sample swaps. We have designed a duplex PCR combining an amplicon from MAOA marking the X chromosome and an amplicon from DDX3Y marking the Y chromosome. We combined this with a simple economical palmtop sized 768-well microplate compatible electrophoresis system developed in-house for examination of duplex PCR products. We applied this quality control test in the validation of two DNA banks.
male, x, research support, electrophoresis, chromosomes, genotype, methods, human, epidemiologic studies, genetics, humans, quality control, dna, combination
3038-3041
Huang, Shuwen
44f28908-02b8-46d7-822c-e6a9e7981723
Chen, Xiao-he
fd93b896-d8cc-4b0e-a811-c0e6a8ff8308
Day, Ian N.M.
b749b30a-1f4c-40eb-af0e-a50427388b39
29 June 2006
Huang, Shuwen
44f28908-02b8-46d7-822c-e6a9e7981723
Chen, Xiao-he
fd93b896-d8cc-4b0e-a811-c0e6a8ff8308
Day, Ian N.M.
b749b30a-1f4c-40eb-af0e-a50427388b39
Huang, Shuwen, Chen, Xiao-he and Day, Ian N.M.
(2006)
Combination of 768-well microplate array diagonal gel electrophoresis with duplex PCR of X and Y chromosome markers for quality control of epidemiological DNA banks.
Electrophoresis, 27 (15), .
(doi:10.1002/elps.200500745).
Abstract
Large DNA banks for human epidemiological studies have become an increasingly important research tool. The power of genotype-phenotype studies is dependent both on the quality of phenotyping and of genotyping and of correct linking of phenotypes to genotypes. Samples must be tracked through numerous steps between subject or patient and post-genotypic data. Only one phenotype, sex, has a perfect and binary correlation with genotype. In mixed sex studies, it may be advantageous for purposes of quality control to keep sexes mixed during the steps from acquisition to DNA bank, in order to be able to check later for sample swaps. We have designed a duplex PCR combining an amplicon from MAOA marking the X chromosome and an amplicon from DDX3Y marking the Y chromosome. We combined this with a simple economical palmtop sized 768-well microplate compatible electrophoresis system developed in-house for examination of duplex PCR products. We applied this quality control test in the validation of two DNA banks.
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Published date: 29 June 2006
Keywords:
male, x, research support, electrophoresis, chromosomes, genotype, methods, human, epidemiologic studies, genetics, humans, quality control, dna, combination
Identifiers
Local EPrints ID: 59866
URI: http://eprints.soton.ac.uk/id/eprint/59866
ISSN: 0173-0835
PURE UUID: 1558e2f4-82b3-4592-9cd5-96920097a013
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Date deposited: 05 Sep 2008
Last modified: 15 Mar 2024 11:18
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Contributors
Author:
Shuwen Huang
Author:
Xiao-he Chen
Author:
Ian N.M. Day
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