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Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury

Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury
Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury
This study evaluates whether cardiac ischemia induces release of urocortin, before and independently from myocyte cell death. Urocortin levels rose after 5-min ischemia and peaked after 10-min ischemia, when cell death was not detected. However, myocyte apoptosis and/or necrosis occurred following 20- and 30-min ischemia, which paralleled a fall in urocortin levels, suggesting that urocortin expression and release are mainly sustained by metabolically challenged, though still viable myocytes. Hence, since cardiac release of urocortin, unlike that of conventional biomarkers, occurs before and apart from cell death, urocortin levels may be clinically useful in the diagnosis of sublethal myocardial ischemia.
molecular biology, London, myocardial ischemia, metabolism, rats, sprague-dawley, diagnosis, injuries, cell death, apoptosis, biology, analysis, pathology, urocortins, male, health, expression, biological markers, heart, animals, necrosis, child, myocardial reperfusion injury
0014-5793
984-990
Knight, R.A.
a48d0c42-7876-4bf9-808b-cfe88d5dfb3c
Chen-Scarabelli, C.
251824ab-74f4-413f-a6bd-5a8bbd38b65e
Yuan, Z.
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McCauley, R.B.
e311a7d4-cec6-4860-8f9a-543b6582f410
Di Rezze, J.
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Scarabelli, G.M.
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Townsend, P.A.
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Latchman, D.
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Saravolatz, L.
b3fd91c8-f321-4a43-ae35-2ba22f6b42ce
Faggian, G.
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Mazzucco, A.
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Chowdrey, H.S.
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Stephanou, A.
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Scarabelli, T. M.
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Knight, R.A.
a48d0c42-7876-4bf9-808b-cfe88d5dfb3c
Chen-Scarabelli, C.
251824ab-74f4-413f-a6bd-5a8bbd38b65e
Yuan, Z.
01944ac8-f3e7-4440-867e-d1a0d2586029
McCauley, R.B.
e311a7d4-cec6-4860-8f9a-543b6582f410
Di Rezze, J.
d4964569-cf76-4057-9f32-2dd9375a5981
Scarabelli, G.M.
10b9b211-65cf-484e-834b-2a7af892244b
Townsend, P.A.
89300833-c898-4ae1-a3b2-03214c71da52
Latchman, D.
0523ed5f-2893-4762-bee1-14d89e2b3cd6
Saravolatz, L.
b3fd91c8-f321-4a43-ae35-2ba22f6b42ce
Faggian, G.
62d4012b-abde-4c84-ad3e-3a4ee6db8baf
Mazzucco, A.
75d6282f-0a90-4404-aac2-a819c4e4684d
Chowdrey, H.S.
3a18f0e6-0913-453f-97b6-fe23773828ed
Stephanou, A.
7481e750-c831-488c-936e-842280541517
Scarabelli, T. M.
78f08b7d-f718-4f1f-aa1d-eda4b186d010

Knight, R.A., Chen-Scarabelli, C., Yuan, Z., McCauley, R.B., Di Rezze, J., Scarabelli, G.M., Townsend, P.A., Latchman, D., Saravolatz, L., Faggian, G., Mazzucco, A., Chowdrey, H.S., Stephanou, A. and Scarabelli, T. M. (2008) Cardiac release of urocortin precedes the occurrence of irreversible myocardial damage in the rat heart exposed to ischemia/reperfusion injury. FEBS Letters, 582 (6), 984-990. (doi:10.1016/j.febslet.2008.02.035).

Record type: Article

Abstract

This study evaluates whether cardiac ischemia induces release of urocortin, before and independently from myocyte cell death. Urocortin levels rose after 5-min ischemia and peaked after 10-min ischemia, when cell death was not detected. However, myocyte apoptosis and/or necrosis occurred following 20- and 30-min ischemia, which paralleled a fall in urocortin levels, suggesting that urocortin expression and release are mainly sustained by metabolically challenged, though still viable myocytes. Hence, since cardiac release of urocortin, unlike that of conventional biomarkers, occurs before and apart from cell death, urocortin levels may be clinically useful in the diagnosis of sublethal myocardial ischemia.

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More information

Published date: 2008
Keywords: molecular biology, London, myocardial ischemia, metabolism, rats, sprague-dawley, diagnosis, injuries, cell death, apoptosis, biology, analysis, pathology, urocortins, male, health, expression, biological markers, heart, animals, necrosis, child, myocardial reperfusion injury

Identifiers

Local EPrints ID: 59935
URI: http://eprints.soton.ac.uk/id/eprint/59935
ISSN: 0014-5793
PURE UUID: f32a987f-88c4-4dc8-a408-bd14009dab20

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Date deposited: 11 Sep 2008
Last modified: 15 Mar 2024 11:18

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Contributors

Author: R.A. Knight
Author: C. Chen-Scarabelli
Author: Z. Yuan
Author: R.B. McCauley
Author: J. Di Rezze
Author: G.M. Scarabelli
Author: P.A. Townsend
Author: D. Latchman
Author: L. Saravolatz
Author: G. Faggian
Author: A. Mazzucco
Author: H.S. Chowdrey
Author: A. Stephanou
Author: T. M. Scarabelli

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