Differences in matrix metalloproteinase-1 and matrix metalloproteinase-12 transcript levels among carotid atherosclerotic plaques with different histopathological characteristics


Morgan, Angharad R., Rerkasem, Kittipan, Gallagher, Patrick J., Zhang, Baiping, Morris, Gareth E., Calder, Philip C., Grimble, Robert F., Eriksson, Per, McPheat, William L., Shearman, Clifford P. and Ye, Shu (2004) Differences in matrix metalloproteinase-1 and matrix metalloproteinase-12 transcript levels among carotid atherosclerotic plaques with different histopathological characteristics. Stroke, 35, (6), 1310-1315. (doi:10.1161/01.STR.0000126822.01756.99).

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Description/Abstract

Background and Purpose— Previous studies have shown that atherosclerotic lesions express a number of matrix metalloproteinases (MMPs). Here we investigated whether transcript levels of MMP-1, -3, -7, -9, and -12 in carotid atherosclerotic plaques were correlated with histological features and clinical manifestations.

Methods— Atherosclerotic plaques (n=50) removed from patients undergoing carotid endarterectomy were classified histologically using a system proposed by Virmani et al, and MMP-1, -3, -7, -9, and -12 transcript levels in these tissues were quantified by real-time reverse-transcriptase polymerase chain reaction.

Results— Compared to plaques with a thick fibrous cap, those with a thin cap had a 7.8-fold higher MMP-1 transcript level (P=0.006). MMP-3, -7, and -12 were 1.5-fold, 1.8-fold, and 2.1-fold, respectively, higher in thin cap plaques, but the differences did not reach statistical significance. MMP-12 transcript levels were significantly increased in ruptured plaques compared with lesions without cap disruption (P=0.001). MMP-9 transcript levels were similar among the different types of lesion. MMP-1 and -12 transcript levels were significantly higher in plaques from patients with amaurosis fugax, than in those from asymptomatic patients (P=0.029 and P=0.008 for MMP-1 and MMP-12, respectively), than in those from patients with stroke (P=0.027 and P=0.001, respectively), and than in those from patients with transient ischemic attack (P=0.046 and P=0.008, respectively).

Conclusions— These data support a role of MMP-1 and -12 in determining atherosclerotic plaque stability.

Item Type: Article
ISSNs: 1524-4628 (print)
Related URLs:
Keywords: aged, role, reverse transcriptase polymerase chain reaction, human, female, metabolism, diagnosis, endarterectomy, male, rna, patients, research support,non-u.s.gov't, genetics, rna, biosynthesis
Subjects: R Medicine
R Medicine > RS Pharmacy and materia medica
Q Science > QR Microbiology
Divisions: University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
University Structure - Pre August 2011 > School of Medicine > Infection, Inflammation and Repair
ePrint ID: 60070
Date Deposited: 08 Sep 2008
Last Modified: 27 Mar 2014 18:42
URI: http://eprints.soton.ac.uk/id/eprint/60070

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