Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography
Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography
It is considered that cells can use filopodia, or microspikes, to locate sites suitable for adhesion. This has been investigated using a number of mature cell types, but, to our knowledge, not progenitor cells. Chemical and topographical cues on the underlying substrate are a useful tool for producing defined features for cells to respond to. In this study, arrays of nanopits with different symmetries (square or hexagonal arrays with 120 nm diameters, 300 nm center-centre spacings) and osteoprogenitor cells were considered. The pits were fabricated by ultra-high precision electron-beam lithography and then reproduced in polycarbonate by injection moulding with a nickel stamp. Using scanning electron and fluorescence microscopies, the initial interactions of the cells via filopodia have been observed, as have subsequent adhesion and cytoskeletal formation. The results showed increased filopodia interaction with the surrounding nanoarchitecture leading to a decrease in cell spreading, focal adhesion formation and cytoskeletal organisation.
80 and over, adult stem cells, physiology, aged, pseudopodia, cell adhesion, bone marrow cells, infection, humans, cytology, female, cues, nanostructures, research
1211-1218
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Wilkinson, Chris D.
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Oreffo, Richard O.
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Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
2007
Hart, Andrew
6011e639-937a-4d81-aa83-ba1ad25ca916
Gadegaard, Nikolaj
bf72c3d1-e6a7-48b4-a968-c496b655bd98
Wilkinson, Chris D.
845f7f7a-6803-408a-b9e7-cd263bc4fe23
Oreffo, Richard O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Hart, Andrew, Gadegaard, Nikolaj, Wilkinson, Chris D., Oreffo, Richard O. and Dalby, Matthew J.
(2007)
Osteoprogenitor response to low-adhesion nanotopographies originally fabricated by electron beam lithography.
Journal of Materials Science: Materials in Medicine, 18 (6), .
(doi:10.1007/s10856-007-0157-7).
Abstract
It is considered that cells can use filopodia, or microspikes, to locate sites suitable for adhesion. This has been investigated using a number of mature cell types, but, to our knowledge, not progenitor cells. Chemical and topographical cues on the underlying substrate are a useful tool for producing defined features for cells to respond to. In this study, arrays of nanopits with different symmetries (square or hexagonal arrays with 120 nm diameters, 300 nm center-centre spacings) and osteoprogenitor cells were considered. The pits were fabricated by ultra-high precision electron-beam lithography and then reproduced in polycarbonate by injection moulding with a nickel stamp. Using scanning electron and fluorescence microscopies, the initial interactions of the cells via filopodia have been observed, as have subsequent adhesion and cytoskeletal formation. The results showed increased filopodia interaction with the surrounding nanoarchitecture leading to a decrease in cell spreading, focal adhesion formation and cytoskeletal organisation.
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Published date: 2007
Keywords:
80 and over, adult stem cells, physiology, aged, pseudopodia, cell adhesion, bone marrow cells, infection, humans, cytology, female, cues, nanostructures, research
Identifiers
Local EPrints ID: 61196
URI: http://eprints.soton.ac.uk/id/eprint/61196
ISSN: 0957-4530
PURE UUID: 73f649d4-2cdf-4570-9133-14fdfe69b767
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Date deposited: 12 Sep 2008
Last modified: 16 Mar 2024 03:11
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Author:
Andrew Hart
Author:
Nikolaj Gadegaard
Author:
Chris D. Wilkinson
Author:
Matthew J. Dalby
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