Maternal 25(OH)-vitamin D status in late pregnancy and mRNA expression of placental calcium transporter predict intrauterine bone mineral accrual in the offspring
Harvey, N.C., Martin, R., Javaid, M.K, Swaminathan, R., Taylor, P., Poole, J.R., Arden, N.K, Dennison, E.M., Inskip, H.M., Godfrey, K., Lewis, R.M. and Cooper, C. (2006) Maternal 25(OH)-vitamin D status in late pregnancy and mRNA expression of placental calcium transporter predict intrauterine bone mineral accrual in the offspring. Osteoporosis International, 17, (Supplement 2), S9 (OC9). (doi:10.1007/s00198-006-0096-z).
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Aims: Evidence suggests that intrauterine bone mineral accrual
predicts osteoporosis risk in later life, and that maternal 25(OH)-
vitamin D status in pregnancy is a determinant of neonatal bone
mass. We aimed to explore the relationship between intrauterine
bone mineral accrual in the offspring, and 1) maternal 25(OH)-
vitamin D status during late pregnancy, and 2) expression of
calcium transporters in the placenta.
Methods: Pregnancies were recruited from the Southampton
Women’s Survey, a unique, ongoing, well established cohort of
women, aged 20–34 years, assessed before and during pregnancy.
Maternal 25(OH)-vitamin D status was measured by radioimmunoassay
in late pregnancy (34 weeks); the healthy, term,
neonates underwent whole body (WB) DXA within 20 days of
birth, using a Lunar DPX instrument. Placental samples, snap
frozen in liquid nitrogen within 30 minutes of birth, were available
for 70 of the pregnancies. A quantitative real time polymerase
chain reaction was used to measure the mRNA expression of
PMCA isoforms 1, 3 and 4 in the placenta, using beta-actin as a
Results: 556 (286 males) neonates were studied. Offspring of
mothers who were deficient (<33nmol/l) in 25(OH)-vitamin D in
late pregnancy had lower bone mass than those of replete mothers.
Thus the mean WB bone area (BA) of the female offspring of
25(OH)-vitamin D deficient mothers was 110cm2 vs 119cm2 in
offspring of replete mothers (p=0.04). The mean WB bone mineral
content (BMC) for offspring of deficient vs replete mothers was
58g vs 63g (p=0.04), respectively. The relationships in the boys did
not reach statistical significance. There was no association with
maternal alkaline phosphatase.
After controlling for beta-actin expression, PMCA3 mRNA
expression predicted neonatal WB BA (r=0.28,p=0.02), WB BMC
(r=0.25,p=0.04), placental weight (r=0.26,p=0.03), and birth
Conclusions: These data are consistent with previous findings
that mothers deficient in 25(OH)-vitamin D in pregnancy have
children with reduced bone mass. The mechanism underlying
this process may be explained, in part, by the demonstrated
association between expression of the placental calcium transporter
PMCA3 and neonatal whole body bone mineral content.
Further elucidation of this process may allow development of
novel therapeutic strategies to optimise childhood bone mineral
accrual and thus reduce osteoporotic fractures in future generations.
|Digital Object Identifier (DOI):||doi:10.1007/s00198-006-0096-z|
|Additional Information:||Oral Communication Abstracts: S133|
|Keywords:||bone, calcium, pregnancy, expression, maternal|
|Subjects:||R Medicine > RA Public aspects of medicine
R Medicine > RF Otorhinolaryngology
|Divisions:||University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
|Date Deposited:||18 Nov 2008|
|Last Modified:||06 Aug 2015 02:49|
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