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Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro

Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro
Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro
The ability to deliver, over time, biologically active vascular endothelial growth factor-165 (VEGF) through tailored designed scaffolds offers tremendous therapeutic opportunities to tissue-engineered therapies. Porous biodegradable poly(DL-lactic) acid (PLA) scaffolds encapsulating VEGF have been generated using supercritical CO2 (scCO2) and the kinetic release and angiogenic activity of these scaffolds examined in vitro and in an ex vivo chick chorioallantoic membrane (CAM) angiogenesis model. After processing through scCO2, VEGF maintained its angiogenic activity as assessed by increased tubule formation of human umbilical vein endothelial cells (HUVEC) cultured on Matrigel (VEGF = 1937 +/- 205 microm; scCO2-VEGF = 2085 +/- 234 microm; control = 1237 +/- 179 microm). VEGF release kinetics from scCO2-VEGF incorporated PLA monolith scaffolds showed a cumulative release of VEGF (2837 +/- 761 rhog/ml) over a 21 day period in culture. In addition, VEGF encapsulated PLA scaffolds increased the blood vessel network in the CAM compared to controls; control, 24.8 +/- 9.6; VEGF/PLA, 44.1 +/- 12.1 (vessels/field). These studies demonstrate that the controlled release of growth factors encapsulated into three-dimensional PLA scaffolds can actively stimulate the rapid development of therapeutic neovascularisation to regenerate or engineer tissues
growth, research, carbon dioxide, acid, umbilical cord, cultured, kinetics, pharmacology, bone, chickens, endothelial cells, therapy, in-vitro, chorioallantoic membrane, cell adhesion, drug effects, animals, polymers, blood, activity, lactic acid, humans, cytology, vascular endothelial growth factor a, blood supply, origins, human, disease, cells, development, health
0006-291X
135-141
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Barry, J.
7c5199dc-34f0-42d0-af45-f4efc9105b45
Ginty, P.
b69ddbe7-9176-4bb0-9224-2f4e0b03ba6a
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Shakesheff, K.M.
9e3f7c5c-9191-40b6-b7b3-f454110a7950
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Kanczler, J.M.
eb8db9ff-a038-475f-9030-48eef2b0559c
Barry, J.
7c5199dc-34f0-42d0-af45-f4efc9105b45
Ginty, P.
b69ddbe7-9176-4bb0-9224-2f4e0b03ba6a
Howdle, S.M.
9aaf52a9-58ae-4811-947a-0498f153cfa5
Shakesheff, K.M.
9e3f7c5c-9191-40b6-b7b3-f454110a7950
Oreffo, R.O.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Kanczler, J.M., Barry, J., Ginty, P., Howdle, S.M., Shakesheff, K.M. and Oreffo, R.O. (2007) Supercritical carbon dioxide generated vascular endothelial growth factor encapsulated poly(DL-lactic acid) scaffolds induce angiogenesis in vitro. Biochemical and Biophysical Research Communications, 352 (1), 135-141.

Record type: Article

Abstract

The ability to deliver, over time, biologically active vascular endothelial growth factor-165 (VEGF) through tailored designed scaffolds offers tremendous therapeutic opportunities to tissue-engineered therapies. Porous biodegradable poly(DL-lactic) acid (PLA) scaffolds encapsulating VEGF have been generated using supercritical CO2 (scCO2) and the kinetic release and angiogenic activity of these scaffolds examined in vitro and in an ex vivo chick chorioallantoic membrane (CAM) angiogenesis model. After processing through scCO2, VEGF maintained its angiogenic activity as assessed by increased tubule formation of human umbilical vein endothelial cells (HUVEC) cultured on Matrigel (VEGF = 1937 +/- 205 microm; scCO2-VEGF = 2085 +/- 234 microm; control = 1237 +/- 179 microm). VEGF release kinetics from scCO2-VEGF incorporated PLA monolith scaffolds showed a cumulative release of VEGF (2837 +/- 761 rhog/ml) over a 21 day period in culture. In addition, VEGF encapsulated PLA scaffolds increased the blood vessel network in the CAM compared to controls; control, 24.8 +/- 9.6; VEGF/PLA, 44.1 +/- 12.1 (vessels/field). These studies demonstrate that the controlled release of growth factors encapsulated into three-dimensional PLA scaffolds can actively stimulate the rapid development of therapeutic neovascularisation to regenerate or engineer tissues

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More information

Published date: January 2007
Keywords: growth, research, carbon dioxide, acid, umbilical cord, cultured, kinetics, pharmacology, bone, chickens, endothelial cells, therapy, in-vitro, chorioallantoic membrane, cell adhesion, drug effects, animals, polymers, blood, activity, lactic acid, humans, cytology, vascular endothelial growth factor a, blood supply, origins, human, disease, cells, development, health

Identifiers

Local EPrints ID: 61272
URI: http://eprints.soton.ac.uk/id/eprint/61272
ISSN: 0006-291X
PURE UUID: 97d8bbe0-daac-4f7d-8353-6bb3eb1684c3
ORCID for J.M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414
ORCID for R.O. Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 04 Sep 2008
Last modified: 11 Jul 2024 01:43

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Contributors

Author: J.M. Kanczler ORCID iD
Author: J. Barry
Author: P. Ginty
Author: S.M. Howdle
Author: K.M. Shakesheff
Author: R.O. Oreffo ORCID iD

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