Maternal low protein diet restricted to the preimplantation period induces a gender-specific change on hepatic gene expression in rat fetuses


Kwong, Wing Yee, Miller, Daniel J., Wilkins, Adrian P., Dear, Mark S., Wright, J. Neville, Osmond, Clive, Zhang, Junlong and Fleming, Tom P. (2007) Maternal low protein diet restricted to the preimplantation period induces a gender-specific change on hepatic gene expression in rat fetuses. Molecular Reproduction and Development, 74, (1), 52-60. (doi:10.1002/mrd.20606).

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Original Publication URL: http://dx.doi.org/10.1002/mrd.20606

Description/Abstract

It has been shown previously that maternal low protein diet (LPD) throughout rat gestation altered hepatic gene expression and enzyme activities in offspring. Here, we investigate the effect of maternal LPD (9% casein vs. 18% control) exclusively during the preimplantation period (switched diet group) or provided throughout gestation on hepatic gene expression in day 20 fetuses. Using quantitative competitive PCR, we found that switched diet induced a two-fold increase (P = 0.008) in hepatic gene expression of phosphoenolpyruvate carboxykinase (PEPCK, a rate limiting enzyme for gluconeogenesis) in male fetuses and a 17% increase (P = 0.005) in 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1, acts primarily as a reductase to produce active glucocorticoid) in female liver compared with control fetuses. Maternal LPD administered throughout gestation increased 11beta-HSD1 expression in male fetal liver by 27% (P = 0.042) compared with controls. However, maternal LPD fed for either period did not affect fetal hepatic insulin receptor (IR), glucocorticoid receptor (GR), glycogen synthase (GS) nor placental glucose transporter 1 (Glut1) and 3 (Glut3) transcript levels. The alteration in fetal hepatic gene expression could not be attributed specifically to known regulators including insulin or glucose concentrations in fetal blood nor alteration in cAMP in fetal liver, although a combination of these regulatory factors may be responsible. Fetal hepatic glycogen level was unaffected by maternal diet. The present findings show that the long term potential of the preimplantation embryo is sensitive to maternal LPD such that basal levels of hepatic gene expression in day 20 fetuses are altered in a gender-specific manner.

Item Type: Article
ISSNs: 1040-452X (print)
Related URLs:
Keywords: phosphoenolpyruvate carboxykinase, 11-hydroxysteroid dehydrogenase type 1, gender
Subjects: R Medicine
Q Science > QH Natural history > QH426 Genetics
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
University Structure - Pre August 2011 > School of Biological Sciences
University Structure - Pre August 2011 > School of Medicine
ePrint ID: 61311
Date Deposited: 30 Sep 2008
Last Modified: 27 Mar 2014 18:43
Contact Email Address: wyk@soton.ac.uk
URI: http://eprints.soton.ac.uk/id/eprint/61311

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