MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage
Söder, S., Roach, H.I., Oehler, S., Bau, B., Haag, J. and Aigner, T. (2006) MMP-9/gelatinase B is a gene product of human adult articular chondrocytes and increased in osteoarthritic cartilage. Clinical and Experimental Rheumatology, 24, (3), 302-304.
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OBJECTIVE: Collagen fibril degeneration involves initially the cleavage within the triple helix by the collagenases 1 (MMP-1) and 3 (MMP-13), but then mainly involves also the gelatinases A (MMP-2) and B (MMP-9). The objective of this study was to determine the quantitative expression levels as well as the distribution in normal and osteoarthritic cartilage of gelatinase B and in cultured articular chondrocytes with and without stimulation by Il-1Beta.
METHODS: Conventional and real-time quantitative PCR technology and immunohistochemistry were used to determine gelatinase B expression on the mRNA and protein level.
RESULTS: Conventional PCR analysis could demonstrate the presence of gelatinase B mRNA only in osteoarthritic chondrocytes. Real-time quantitative PCR confirmed the increased expression of gelatinase B mRNA expression in osteoarthritic chondrocytes. No significant up-regulation of gelatinase B was observed by Il-1Beta. Immunostaining for gelatinase B showed the presence of gelatinase B in a subset of normal and in a large portion of osteoarthritic chondrocytes with a more extended distribution in the latter.
CONCLUSION: In osteoarthritic cartilage destruction, gelatinase B is involved in collagen destruction though still at a very much lower level than gelatinase A. Only a very small subset of normal adult articular chondrocytes express gelatinase B in vivo suggesting that gelatinase B unlike gelatinase A is hardly or only very focally involved in physiological collagen turnover.
|Keywords:||immunohistochemistry, messenger, gene expression, middle aged, human, rna, cytology, reverse transcriptase polymerase chain reaction, gene, adult, osteoarthritis, genetics, expression, knee, collagen, cultured, chondrocytes, cartilage, pharmacology, protein, pathology, cells, drug, interleukin-1, aged, metabolism, analysis, articular, 80 and over, humans, drug effects, aged, rna, methods, research, in-vivo, femur head, biological markers, dose-response relationship, matrix metalloproteinase 9, up-regulation, germany|
|Subjects:||R Medicine > RC Internal medicine
Q Science > QP Physiology
Q Science > QH Natural history > QH426 Genetics
|Divisions:||University Structure - Pre August 2011 > School of Medicine
|Date Deposited:||16 Jun 2009|
|Last Modified:||02 Mar 2012 11:32|
|Contributors:||Söder, S. (Author)
Roach, H.I. (Author)
Oehler, S. (Author)
Bau, B. (Author)
Haag, J. (Author)
Aigner, T. (Author)
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