Evaluation of human bone marrow stromal cell growth on biodegradable polymer/Bioglass composites
Evaluation of human bone marrow stromal cell growth on biodegradable polymer/Bioglass composites
Bone tissue engineering using human bone marrow mesenchymal stem cells (HBMCs) and biocompatible materials provides an attractive approach to regenerate bone tissue to meet the major clinical need. The aim of this study was to examine the effects of novel porous biodegradable composite materials consisting of a bioactive phase (45S5 Bioglass, 0, 5, and 40 wt%) incorporated within a biodegradable poly(dl-lactic acid) matrix, on HBMCs growth. Cell adhesion, spreading, and viability was examined using Cell Tracker Green/Ethidium Homodimer-1. Bone formation was assessed using scaffolds seeded with stro-1 positive HBMCs in nude mice. In vitro biochemistry indicated that with minimal scaffold pre-treatment osteoblast activity falls with increasing Bioglass content. However, 24h scaffold pre-treatment with serum resulted in a significant increase in alkaline phosphatase specific activity in 5 wt% Bioglass composites relative to the 0 and 40 wt% Bioglass groups. In vivo studies indicate significant new bone formation throughout all the scaffolds, as evidenced by immunohistochemistry.
bone marrow, cells, growth, alkaline phosphatase, mice, cell adhesion, acid, immunohistochemistry, bone, biocompatible materials, mesenchymal stem cells, in-vivo, human, in-vitro, activity, tissue engineering, stem cells
1098-1107
Yang, Xuebin B.
70deb9e6-145c-46f8-9f57-c20de4cd803c
Webb, Derek
24224390-0228-4bec-a5a0-87431f0405e6
Blaker, Jonny
335d1dd7-e240-4e23-b026-d9c99d159876
Boccaccini, Aldo R.
847415b9-fdd9-41c0-acb7-3806e53fcabd
Maquet, Veronique
1bdeb4b1-1089-40e6-9660-54a62d90cec1
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Oreffo, Richard O.C.
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21 April 2006
Yang, Xuebin B.
70deb9e6-145c-46f8-9f57-c20de4cd803c
Webb, Derek
24224390-0228-4bec-a5a0-87431f0405e6
Blaker, Jonny
335d1dd7-e240-4e23-b026-d9c99d159876
Boccaccini, Aldo R.
847415b9-fdd9-41c0-acb7-3806e53fcabd
Maquet, Veronique
1bdeb4b1-1089-40e6-9660-54a62d90cec1
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Yang, Xuebin B., Webb, Derek, Blaker, Jonny, Boccaccini, Aldo R., Maquet, Veronique, Cooper, Cyrus and Oreffo, Richard O.C.
(2006)
Evaluation of human bone marrow stromal cell growth on biodegradable polymer/Bioglass composites.
Biochemical and Biophysical Research Communications, 342 (4), .
(doi:10.1016/j.bbrc.2006.02.021).
Abstract
Bone tissue engineering using human bone marrow mesenchymal stem cells (HBMCs) and biocompatible materials provides an attractive approach to regenerate bone tissue to meet the major clinical need. The aim of this study was to examine the effects of novel porous biodegradable composite materials consisting of a bioactive phase (45S5 Bioglass, 0, 5, and 40 wt%) incorporated within a biodegradable poly(dl-lactic acid) matrix, on HBMCs growth. Cell adhesion, spreading, and viability was examined using Cell Tracker Green/Ethidium Homodimer-1. Bone formation was assessed using scaffolds seeded with stro-1 positive HBMCs in nude mice. In vitro biochemistry indicated that with minimal scaffold pre-treatment osteoblast activity falls with increasing Bioglass content. However, 24h scaffold pre-treatment with serum resulted in a significant increase in alkaline phosphatase specific activity in 5 wt% Bioglass composites relative to the 0 and 40 wt% Bioglass groups. In vivo studies indicate significant new bone formation throughout all the scaffolds, as evidenced by immunohistochemistry.
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Published date: 21 April 2006
Keywords:
bone marrow, cells, growth, alkaline phosphatase, mice, cell adhesion, acid, immunohistochemistry, bone, biocompatible materials, mesenchymal stem cells, in-vivo, human, in-vitro, activity, tissue engineering, stem cells
Identifiers
Local EPrints ID: 61624
URI: http://eprints.soton.ac.uk/id/eprint/61624
ISSN: 0006-291X
PURE UUID: 39e903b2-895a-4acf-ab43-a4f0946300f0
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Date deposited: 30 Mar 2009
Last modified: 18 Mar 2024 02:51
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Contributors
Author:
Xuebin B. Yang
Author:
Derek Webb
Author:
Jonny Blaker
Author:
Aldo R. Boccaccini
Author:
Veronique Maquet
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