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An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures

An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures
An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures
Traumatic brain injury (TBI) is caused by rapid deformation of the brain, resulting in a cascade of pathological events and ultimately neurodegeneration. Understanding how the biomechanics of brain deformation leads to tissue damage remains a considerable challenge. We have developed an in vitro model of TBI utilising organotypic hippocampal slice cultures on deformable silicone membranes, and an injury device, which generates tissue deformation through stretching the silicone substrate. Our injury device controls the biomechanical parameters of the stretch via feedback control, resulting in a reproducible and equi-biaxial deformation stimulus. Organotypic cultures remain well adhered to the membrane during deformation, so that tissue strain is 93 and 86% of the membrane strain in the x- and y-axis, respectively. Cell damage following injury is positively correlated with strain. In conclusion, we have developed a unique in vitro model to study the effects of mechanical stimuli within a complex cellular environment that mimics the in vivo environment. We believe this model could be a powerful tool to study the acute phases of TBI and the induced cell degeneration could provide a good platform for the development of potential therapeutic approaches and may be a useful in vitro alternative to animal models of TBI.
slice cultures, deformation, head-injury, injuries, neurons, mechanical stretch, damage, mechanical injury, model, brain, cells, brain-injury, tissue, diffuse axonal injury, neurodegeneration
0165-0270
192-201
Morrison, Barclay
97d2b4dc-0ce1-4706-a0ee-bb3ce7733f22
Cater, Heather L.
39c5b10e-e778-4d54-ab5e-29d76b82c343
Benham, Christopher D.
84eb1cf3-7c2a-4f6e-b661-af58b9262589
Sundstrom, Lars E.
bb62018d-0157-4274-a865-448ed12934bd
Morrison, Barclay
97d2b4dc-0ce1-4706-a0ee-bb3ce7733f22
Cater, Heather L.
39c5b10e-e778-4d54-ab5e-29d76b82c343
Benham, Christopher D.
84eb1cf3-7c2a-4f6e-b661-af58b9262589
Sundstrom, Lars E.
bb62018d-0157-4274-a865-448ed12934bd

Morrison, Barclay, Cater, Heather L., Benham, Christopher D. and Sundstrom, Lars E. (2006) An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures. Journal of Neuroscience Methods, 150 (2), 192-201. (doi:10.1016/j.jneumeth.2005.06.014).

Record type: Article

Abstract

Traumatic brain injury (TBI) is caused by rapid deformation of the brain, resulting in a cascade of pathological events and ultimately neurodegeneration. Understanding how the biomechanics of brain deformation leads to tissue damage remains a considerable challenge. We have developed an in vitro model of TBI utilising organotypic hippocampal slice cultures on deformable silicone membranes, and an injury device, which generates tissue deformation through stretching the silicone substrate. Our injury device controls the biomechanical parameters of the stretch via feedback control, resulting in a reproducible and equi-biaxial deformation stimulus. Organotypic cultures remain well adhered to the membrane during deformation, so that tissue strain is 93 and 86% of the membrane strain in the x- and y-axis, respectively. Cell damage following injury is positively correlated with strain. In conclusion, we have developed a unique in vitro model to study the effects of mechanical stimuli within a complex cellular environment that mimics the in vivo environment. We believe this model could be a powerful tool to study the acute phases of TBI and the induced cell degeneration could provide a good platform for the development of potential therapeutic approaches and may be a useful in vitro alternative to animal models of TBI.

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More information

Published date: 30 January 2006
Keywords: slice cultures, deformation, head-injury, injuries, neurons, mechanical stretch, damage, mechanical injury, model, brain, cells, brain-injury, tissue, diffuse axonal injury, neurodegeneration

Identifiers

Local EPrints ID: 62505
URI: http://eprints.soton.ac.uk/id/eprint/62505
ISSN: 0165-0270
PURE UUID: 8aa54d7e-adf6-4f59-a564-55043d5c3158

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Date deposited: 12 Sep 2008
Last modified: 15 Mar 2024 11:31

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Contributors

Author: Barclay Morrison
Author: Heather L. Cater
Author: Christopher D. Benham
Author: Lars E. Sundstrom

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