An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide
Glynne-Jones, Peter, Hill, Martyn, Townsend, Rosemary J., Harris, Nicholas R., Wilkinson, James S., Zhang, Fan and Melvin, Tracy (2008) An integrated multimodal acoustic particle manipulator and optical evanescent field waveguide. Journal of the Acoustical Society of America, 123, (5), p.3925. (doi:10.1121/1.2935963).
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A new acoustic-optical-microfluidic system is presented for the manipulation of bead-tagged DNA molecules. Acoustic radiation forces are used to manipulate microspheres into and away from the evanescent field of a laser coupled waveguide that is integrated into the reflector of the acoustic chamber. With suitable fluorophores the presence of the target DNA can be detected with a fluorescence microscope enabling large populations of beads to be examined simultaneously. The integrated waveguide and multimodal acoustic chamber are presented here, with results showing that the microspheres can be successfully detected as they are brought into the evanescent field using a quarter-wave acoustic configuration. It is also shown that by measuring the time of flight of a microsphere between the half- and quarterwave nodal planes the bead size can be determined, providing a means of multiplexing the detection (detecting a range of different target DNA sequences).
|Subjects:||T Technology > TK Electrical engineering. Electronics Nuclear engineering
Q Science > QH Natural history > QH301 Biology
Q Science > QC Physics
|Divisions:||University Structure - Pre August 2011 > School of Engineering Sciences
University Structure - Pre August 2011 > School of Electronics and Computer Science
University Structure - Pre August 2011 > Optoelectronics Research Centre
|Date Deposited:||08 Oct 2008|
|Last Modified:||15 Oct 2012 15:07|
|Contributors:||Glynne-Jones, Peter (Author)
Hill, Martyn (Author)
Townsend, Rosemary J. (Author)
Harris, Nicholas R. (Author)
Wilkinson, James S. (Author)
Zhang, Fan (Author)
Melvin, Tracy (Author)
|Contact Email Address:||firstname.lastname@example.org|
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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