Discrimination and analysis of phytoplankton using a microfluidic cytometer
Benazzi, G., Holmes, D., Sun, T., Mowlem, M.C. and Morgan, H. (2007) Discrimination and analysis of phytoplankton using a microfluidic cytometer. IET Nanobiotechnology, 1, (6), 94-101. (doi:10.1049/iet-nbt:20070020).
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Identification and analysis of phytoplankton is important for understanding the environmental
parameters that are influenced by the oceans, including pollution and climate change.
Phytoplanktons are studied at the single cell level using conventional light-field and fluorescence
microscopy, but the technique is labour intensive. Flow cytometry enables rapid and quantitative
measurements of single cells and is now used as an analytical tool in phytoplankton analysis.
However, it has a number of drawbacks, including high cost and portability. We describe the fabrication
of a microfluidic (lab-on-a-chip) device for high-speed analysis of single phytoplankton.
The device measures fluorescence (at three wavelength ranges) and the electrical impedance of
single particles. The system was tested using a mixture of three algae (Isochrysis Galbana,
Rhodosorus m., Synechococcus sp.) and the results compared with predictions from theory and
measurements using a commercial flow cytometer (BD FACSAria). It is shown that the microfluidic
flow cytometer is able to distinguish and characterise these different taxa and that impedance
spectroscopy enables measurement of phytoplankton biophysical properties.
|Subjects:||T Technology > TK Electrical engineering. Electronics Nuclear engineering
G Geography. Anthropology. Recreation > GC Oceanography
|Divisions:||University Structure - Pre August 2011 > School of Electronics and Computer Science
University Structure - Pre August 2011 > National Oceanography Centre (NERC)
|Date Deposited:||18 Nov 2008|
|Last Modified:||14 May 2013 01:02|
|Contributors:||Benazzi, G. (Author)
Holmes, D. (Author)
Sun, T. (Author)
Mowlem, M.C. (Author)
Morgan, H. (Author)
|RDF:||RDF+N-Triples, RDF+N3, RDF+XML, Browse.|
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