Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays
Yin, Huabing, Pattrick, Nicola, Zhang, Xunli, Klauke, Norbert, Cordingley, Hayley C., Haswell, Steven J. and Cooper, Jonathan M. (2008) Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. Analytical Chemistry, 80, (1), 179-185. (doi:10.1021/ac701958z).
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Description/Abstract
In this paper, we compare a quantitative cell-based assay measuring the intracellular Ca2+ response to the agonist uridine 5‘-triphosphate in Chinese hamster ovary cells, in both microfluidic and microtiter formats. The study demonstrates that, under appropriate hydrodynamic conditions, there is an excellent agreement between traditional well-plate assays and those obtained on-chip for both suspended immobilized cells and cultured adherent cells. We also demonstrate that the on-chip assay, using adherent cells, provides the possibility of faster screening protocols with the potential for resolving subcelluar information about local Ca2+ flux.
| Item Type: | Article |
|---|---|
| ISSNs: | 0003-2700 (print) |
| Related URLs: | |
| Subjects: | Q Science > QH Natural history > QH301 Biology T Technology > TP Chemical technology |
| Divisions: | University Structure - Pre August 2011 > School of Engineering Sciences > Bioengineering Sciences |
| Item ID: | 64826 |
| Date Deposited: | 16 Jan 2009 |
| Last Modified: | 01 Jun 2012 15:30 |
| Contributors: | Yin, Huabing (Author) Pattrick, Nicola (Author) Zhang, Xunli (Author) Klauke, Norbert (Author) Cordingley, Hayley C. (Author) Haswell, Steven J. (Author) Cooper, Jonathan M. (Author) |
| Date: | 1 January 2008 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/64826 |
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Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. (deposited 09 Feb 2009)
- Quantitative comparison between microfluidic and microtiter plate formats for cell-based assays. (deposited 16 Jan 2009) [Currently Displayed]
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