Interpretation of fast repetition rate (FRR) fluorescence: signatures of phytoplankton community structure versus physiological state


Suggett, David J., Moore, C. Mark, Hickman, A and Geider, Richard J. (2009) Interpretation of fast repetition rate (FRR) fluorescence: signatures of phytoplankton community structure versus physiological state. Marine Ecology Progress Series, 376, 1-19. (doi:10.3354/meps07830).

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Description/Abstract

Introduction of active chlorophyll a fluorescence
protocols, in particular fast repetition rate
(FRR) fluorometry, to oceanography and limnology
15 yr ago has enabled rapid assessment of photosynthetic
physiology in situ. The FRR protocol generates
simultaneous measurements of Photosystem II (PSII)
effective absorption cross sections (termed σPSII) and
photochemical efficiency (termed Fv/Fm). Both Fv/Fm
and σPSII measurements have been utilised to examine
the effects of physiological stress on the photosynthetic
apparatus of phytoplankton in an ever growing number
of fluorescence-based studies. However, it is now
becoming clearer that in situ values of Fv/Fm and σPSII
also contain taxonomic information. Here, we present
a synthesis of previously unpublished and published
data, which show that Fv/Fm and σPSII vary principally
with broad-scale changes in community structure.
These patterns observed in situ conform to trends
observed in laboratory-grown cultures of a range of
phytoplankton taxa. The magnitudes of variability in
Fv/Fm and σPSII driven by changes in phytoplankton
community structure often exceed that induced by
nutrient limitation (as determined from controlled
nutrient addition experiments). An exception to this
general trend occurs in high-nutrient, low-chlorophyll
a (HNLC) regions, where strong phenotypic changes
in Fv/Fm and σPSII have been repeatedly demonstrated
on relief of iron limitation. Overall, FRR fluorescence
measurements of both Fv/Fm and σPSII in natural populations
represent a combination of the taxonomic ‘signature’
(values of Fv/Fm and σPSII determined by the
taxa present) within the phytoplankton community
that is further modified according to the (photo-) physiological
status. As such, fluorescence-based investigations
of mixed populations must account for potential
variations in phytoplankton community structure before
interpretations of physiological status are made.

Item Type: Article
ISSNs: 0171-8630 (print)
Related URLs:
Keywords: Fast repetition rate, Fluorescence, PSII, Effective absorption cross section, Photochemical efficiency, Phytoplankton, Nutrient stress
Subjects: G Geography. Anthropology. Recreation > GC Oceanography
Q Science > QH Natural history > QH301 Biology
Divisions: University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
ePrint ID: 65686
Date Deposited: 09 Mar 2009
Last Modified: 27 Mar 2014 18:47
Projects:
The biophysical basis of iron-light co-limitation of phytoplankton photosynthesis
Funded by: NERC (NE/C518114/2)
Led by: Christopher Mark Moore
1 July 2007 to 31 December 2008
URI: http://eprints.soton.ac.uk/id/eprint/65686

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