E-Cadherin regulates neural stem cell self-renewal


Karpowicz, P., Willaime-Morawek, S., Balenci, L., DeVeale, B., Inoue, T. and van der Kooy, D. (2009) E-Cadherin regulates neural stem cell self-renewal. Journal of Neuroscience, 29, (12), 3885-3896. (doi:10.1523/JNEUROSCI.0037-09.2009).

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Description/Abstract

E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells.

Item Type: Article
ISSNs: 0270-6474 (print)
Related URLs:
Subjects: R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Q Science > QH Natural history > QH426 Genetics
Divisions: University Structure - Pre August 2011 > School of Medicine > Clinical Neurosciences
ePrint ID: 66445
Date Deposited: 15 Jun 2009
Last Modified: 27 Mar 2014 18:47
URI: http://eprints.soton.ac.uk/id/eprint/66445

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