E-Cadherin regulates neural stem cell self-renewal
E-Cadherin regulates neural stem cell self-renewal
E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells.
3885-3896
Karpowicz, P.
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Willaime-Morawek, S.
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Balenci, L.
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DeVeale, B.
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Inoue, T.
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van der Kooy, D.
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25 March 2009
Karpowicz, P.
1bccd0dc-6b19-489d-8268-f8ceb91f12e1
Willaime-Morawek, S.
24a2981f-aa9e-4bf6-ad12-2ccf6b49f1c0
Balenci, L.
d5b43f8e-51bc-4e70-a865-2ccd9e205795
DeVeale, B.
2f950d69-a0e2-42bf-9ea0-f39a4d26b877
Inoue, T.
b2b464ee-530b-47dc-a783-305741bee5c3
van der Kooy, D.
b9a759b8-56ec-4059-9421-02337257fa16
Karpowicz, P., Willaime-Morawek, S., Balenci, L., DeVeale, B., Inoue, T. and van der Kooy, D.
(2009)
E-Cadherin regulates neural stem cell self-renewal.
Journal of Neuroscience, 29 (12), .
(doi:10.1523/JNEUROSCI.0037-09.2009).
Abstract
E-Cadherin, a cell adhesion protein, has been shown to take part in the compartmentalization, proliferation, survival, and differentiation of cells. E-Cadherin is expressed in the adult and embryonic forebrain germinal zones in vivo, and in clonal colonies of cells derived from these regions and grown in vitro. Mice carrying E-Cadherin floxed genes crossed to mice expressing Cre under the Nestin promoter demonstrate defects in the self-renewal of neural stem cells both in vivo and in vitro. The functional role of E-Cadherin is further demonstrated using adhesion-blocking antibodies in vitro, which specifically target cadherin extracellular adhesive domains. Adult neural stem cell colonies decrease in the presence of E-Cadherin antibodies in a dosage-dependent manner, in contrast to P-Cadherin antibody. On overexpression of normal E-Cadherin and a mutated E-Cadherin, containing no intracellular binding domain, an increased number of clonal adult neural stem cell colonies are observed. These data suggest it is specifically E-Cadherin adhesion that is responsible for these self-renewal effects. These data show the importance of E-Cadherin in the neural stem cell niche and suggest E-Cadherin regulates the number of these cells.
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Published date: 25 March 2009
Organisations:
Clinical Neurosciences
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Local EPrints ID: 66445
URI: http://eprints.soton.ac.uk/id/eprint/66445
ISSN: 0270-6474
PURE UUID: ed50a39a-dbbf-4c13-9a83-492246e39794
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Date deposited: 15 Jun 2009
Last modified: 14 Mar 2024 02:53
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Author:
P. Karpowicz
Author:
L. Balenci
Author:
B. DeVeale
Author:
T. Inoue
Author:
D. van der Kooy
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