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Chronological expression of ciliated bronchial epithelium 1 during pulmonary development

Chronological expression of ciliated bronchial epithelium 1 during pulmonary development
Chronological expression of ciliated bronchial epithelium 1 during pulmonary development
Ciliated Bronchial Epithelium (CBE) 1 is a novel gene, which is expressed in ciliated cells. As cilia are important during embryogenesis, the present authors characterised the murine homologue of CBE1 (Cbe1) and compared its temporal expression during murine and human lung development. 
Cbe1 cDNA was cloned and characterised using sequencing, standard PCR and Western blotting. Mouse and human embryonic/fetal lungs (HELs) were harvested for mRNA analysis and protein localisation in vivo and in vitro using RT-PCR and immunohistochemistry.
The Cbe1 amino acid sequence was >75% identical with CBE1 and its alternative splicing and tissue distribution were highly conserved. Pulmonary expression of Cbe1 mRNA was increased at embryonic day (E)16, 1 day later than Foxj1, which is consistent with a role in ciliogenesis. In HELs, CBE1 mRNA was detectable at 8–9 weeks post-conception and increased in explant culture. CBE1 protein expression was weak at 10 weeks post-conception but strong at 12.3 weeks post-conception, in parallel with cilia formation. Additionally, Cbe1 mRNA was expressed at E11 (4–5 weeks post-conception in HELs) in the absence of Foxj1, implying a distinct role in early development. 
Chronological regulation of CBE1/Cbe1 expression during pulmonary differentiation suggests involvement in ciliogenesis, with an additional role during early lung development.
0903-1936
1095-1104
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Yoshisue, H.
b3871d77-c0aa-4265-9fea-b3f11ce43f11
Ribbene, A.
dffbd65c-7c58-4cab-84e5-f21658df866f
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Bucchieri, F.
830cf621-3e39-42ab-b0ea-7d5dc43bf032
Hanley, N.A.
ac5bcdcc-51dd-4f2c-ad06-fef6c1d50ebd
Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Zummo, G.
c8cc057b-11ed-4e33-a49c-dd6e07f2ce49
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Haitchi, H.M.
68dadb29-305d-4236-884f-e9c93f4d78fe
Yoshisue, H.
b3871d77-c0aa-4265-9fea-b3f11ce43f11
Ribbene, A.
dffbd65c-7c58-4cab-84e5-f21658df866f
Wilson, S.J.
21c6875d-6870-441b-ae7a-603562a646b8
Holloway, J.W.
4bbd77e6-c095-445d-a36b-a50a72f6fe1a
Bucchieri, F.
830cf621-3e39-42ab-b0ea-7d5dc43bf032
Hanley, N.A.
ac5bcdcc-51dd-4f2c-ad06-fef6c1d50ebd
Wilson, D.I.
1500fca1-7082-4271-95f4-691f1d1252a2
Zummo, G.
c8cc057b-11ed-4e33-a49c-dd6e07f2ce49
Holgate, S.T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Davies, D.E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38

Haitchi, H.M., Yoshisue, H., Ribbene, A., Wilson, S.J., Holloway, J.W., Bucchieri, F., Hanley, N.A., Wilson, D.I., Zummo, G., Holgate, S.T. and Davies, D.E. (2009) Chronological expression of ciliated bronchial epithelium 1 during pulmonary development. European Respiratory Journal, 33 (5), 1095-1104. (doi:10.1183/09031936.00157108).

Record type: Article

Abstract

Ciliated Bronchial Epithelium (CBE) 1 is a novel gene, which is expressed in ciliated cells. As cilia are important during embryogenesis, the present authors characterised the murine homologue of CBE1 (Cbe1) and compared its temporal expression during murine and human lung development. 
Cbe1 cDNA was cloned and characterised using sequencing, standard PCR and Western blotting. Mouse and human embryonic/fetal lungs (HELs) were harvested for mRNA analysis and protein localisation in vivo and in vitro using RT-PCR and immunohistochemistry.
The Cbe1 amino acid sequence was >75% identical with CBE1 and its alternative splicing and tissue distribution were highly conserved. Pulmonary expression of Cbe1 mRNA was increased at embryonic day (E)16, 1 day later than Foxj1, which is consistent with a role in ciliogenesis. In HELs, CBE1 mRNA was detectable at 8–9 weeks post-conception and increased in explant culture. CBE1 protein expression was weak at 10 weeks post-conception but strong at 12.3 weeks post-conception, in parallel with cilia formation. Additionally, Cbe1 mRNA was expressed at E11 (4–5 weeks post-conception in HELs) in the absence of Foxj1, implying a distinct role in early development. 
Chronological regulation of CBE1/Cbe1 expression during pulmonary differentiation suggests involvement in ciliogenesis, with an additional role during early lung development.

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Published date: May 2009

Identifiers

Local EPrints ID: 69697
URI: http://eprints.soton.ac.uk/id/eprint/69697
ISSN: 0903-1936
PURE UUID: c2931ca5-f633-44a4-a182-d0cd5c30ef4f
ORCID for H.M. Haitchi: ORCID iD orcid.org/0000-0001-8603-302X
ORCID for S.J. Wilson: ORCID iD orcid.org/0000-0003-1305-8271
ORCID for J.W. Holloway: ORCID iD orcid.org/0000-0001-9998-0464
ORCID for D.E. Davies: ORCID iD orcid.org/0000-0002-5117-2991

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Date deposited: 30 Nov 2009
Last modified: 14 Mar 2024 02:47

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Contributors

Author: H.M. Haitchi ORCID iD
Author: H. Yoshisue
Author: A. Ribbene
Author: S.J. Wilson ORCID iD
Author: J.W. Holloway ORCID iD
Author: F. Bucchieri
Author: N.A. Hanley
Author: D.I. Wilson
Author: G. Zummo
Author: S.T. Holgate
Author: D.E. Davies ORCID iD

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