Multi-modal particle manipulator to enhance bead-based bioassays


Glynne-Jones, P., Boltryk, R.J., Hill, M., Zhang, F., Dong, L., Wilkinson, J.S., Brown, T., Melvin, T. and Harris, N.R. (2010) Multi-modal particle manipulator to enhance bead-based bioassays. Ultrasonics, 50, (2), 235-239. (doi:10.1016/j.ultras.2009.09.025).

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Description/Abstract

By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalised glass surface and polystyrene micro-beads, and identify those that bind to the surface by illuminating bound beads using an evanescent field generated by guided light. The movement towards and immobilisation of streptavidin coated beads onto a biotin functionalised waveguide surface is achieved by using a quarter-wavelength mode pushing beads onto the surface, while the removal of non-specifically bound beads uses a second quarter-wavelength mode which exhibits a kinetic energy maximum at the boundary between the carrier layer and fluid, drawing beads towards this surface. This has been achieved using a multi-modal acoustic device which exhibits both of these quarter-wavelength resonances. Both 1-D acoustic modelling and finite element analysis has been used to design this device and to investigate the spatial uniformity of the field. We demonstrate experimentally that 90% of specifically bound beads remain attached after applying ultrasound, with 80% of non-specifically bound control beads being successfully removed acoustically. This approach overcomes problems associated with lengthy sedimentation processes used for bead-based bioassays and surface (electrostatic) forces, which delay or prevent immobilisation. We explain the potential of this technique in the development of DNA and protein assays in terms of detection speed and multiplexing

Item Type: Article
ISSNs: 0041-624X (print)
1874-9968 (electronic)
Related URLs:
Keywords: acoustic radiation forces, bio-sensor, frequency switching, micro-beads, optical waveguide
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering
T Technology > TP Chemical technology
Divisions: University Structure - Pre August 2011 > School of Engineering Sciences > Electro-Mechanical Engineering
Faculty of Physical Sciences and Engineering > Electronics and Computer Science
Faculty of Physical Sciences and Engineering > Optoelectronics Research Centre
ePrint ID: 69843
Date Deposited: 08 Dec 2009
Last Modified: 27 Mar 2014 18:49
Contact Email Address: m.hill@soton.ac.uk
Further Information:Google Scholar
ISI Citation Count:6
URI: http://eprints.soton.ac.uk/id/eprint/69843

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