Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance
Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype
bone, expression, life, protein, human, phenotype, analysis, proteins, mass, bone marrow
1137-1146
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Burchmore, Richard
967325b6-3596-4de0-be59-01ea5f3eaa71
Wilkinson, Chris D.W.
ea556659-2ba1-43a8-b8d3-37e4bfaffd41
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
2008
Kantawong, Fahsai
d209298e-ac00-49f0-a9b9-39d1e82d5925
Burchmore, Richard
967325b6-3596-4de0-be59-01ea5f3eaa71
Wilkinson, Chris D.W.
ea556659-2ba1-43a8-b8d3-37e4bfaffd41
Oreffo, Richard O.C.
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Kantawong, Fahsai, Burchmore, Richard, Wilkinson, Chris D.W., Oreffo, Richard O.C. and Dalby, Matthew J.
(2008)
Differential in-gel electrophoresis (DIGE) analysis of human bone marrow osteoprogenitor cell contact guidance.
Acta Biomaterialia, 5 (4), .
(doi:10.1016/j.actbio.2008.11.001).
Abstract
We have used a recent comparative proteomics technique, differential in-gel electrophoresis (DIGE), to study osteoprogenitor cell response to contact guidance in grooves. In order to increase protein output from small sample sizes, we used bioreactor culture before protein extraction and gel electrophoresis. Mass spectroscopy was used for protein identification. A number of distinct proteins were observed to exhibit significant changes in expression. These changes in protein expression suggest that the cells respond to tailored grooved topographies, with alterations in their proteome concurrent with changes in osteoprogenitor phenotype
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Published date: 2008
Keywords:
bone, expression, life, protein, human, phenotype, analysis, proteins, mass, bone marrow
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Local EPrints ID: 70442
URI: http://eprints.soton.ac.uk/id/eprint/70442
ISSN: 1742-7061
PURE UUID: 620d24c5-4665-464c-820e-d0567bc40f2a
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Date deposited: 10 Feb 2010
Last modified: 14 Mar 2024 02:43
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Author:
Fahsai Kantawong
Author:
Richard Burchmore
Author:
Chris D.W. Wilkinson
Author:
Matthew J. Dalby
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