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Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis

Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis
Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis
Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a multisystem disease, the pathogenesis of which remains undetermined. We set out to determine the precise abnormalities of gene expression in the blood of patients with CFS/ME. We analyzed gene expression in peripheral blood from 25 patients with CFS/ME diagnosed according to the Centers for Disease Control and Prevention diagnostic criteria and 50 healthy blood donors, using a microarray with a cutoff fold difference of expression of >or=2.5. Genes showing differential expression were further analyzed in 55 patients with CFS/ME and 75 healthy blood donors, using quantitative polymerase chain reaction. Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downregulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reaction data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.
0022-1899
1171-1184
Kerr, Jonathan R.
bade6a01-b43f-497b-88ad-4cfd3106a4e0
Petty, Robert
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Burke, Beverley
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Gough, John
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Fear, David
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Sinclair, Lindsey I.
620f952b-3e25-4d66-86d3-04ce73c7330d
Mattey, Derek L.
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Richards, Selwyn C.M.
e43b195b-592e-400a-9ce3-9f38a2a31953
Montgomery, Jane
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Baldwin, Don A.
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Kellam, Paul
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Harrison, Tim J.
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Griffin, George E.
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Main, Janice
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Enlander, Derek
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Nutt, David J.
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Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc
Kerr, Jonathan R.
bade6a01-b43f-497b-88ad-4cfd3106a4e0
Petty, Robert
0905fac6-1bf5-46f2-a924-83188b27624d
Burke, Beverley
6d3f2dd9-2ba2-460c-8405-8ea89dcad985
Gough, John
62c4f9ca-3036-4835-be5b-cf4103494b9d
Fear, David
eab7dbf5-a69c-4ddb-aa57-201b65d2e129
Sinclair, Lindsey I.
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Mattey, Derek L.
16b6b36c-7b2c-4a56-8104-92d3f1dd08a4
Richards, Selwyn C.M.
e43b195b-592e-400a-9ce3-9f38a2a31953
Montgomery, Jane
0858ec3c-54af-4778-82d2-1aa5e5d464f0
Baldwin, Don A.
c0ddf5e3-53f3-4343-a70a-367d4f42bad3
Kellam, Paul
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Harrison, Tim J.
30131407-ba36-4e9a-bb77-ff362fdda91d
Griffin, George E.
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Main, Janice
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Enlander, Derek
7be07d85-bbad-4a58-ac2d-0974716a648c
Nutt, David J.
d0ea03e2-172d-4888-bfd0-239dc7c953cb
Holgate, Stephen T.
2e7c17a9-6796-436e-8772-1fe6d2ac5edc

Kerr, Jonathan R., Petty, Robert, Burke, Beverley, Gough, John, Fear, David, Sinclair, Lindsey I., Mattey, Derek L., Richards, Selwyn C.M., Montgomery, Jane, Baldwin, Don A., Kellam, Paul, Harrison, Tim J., Griffin, George E., Main, Janice, Enlander, Derek, Nutt, David J. and Holgate, Stephen T. (2008) Gene expression subtypes in patients with chronic fatigue syndrome/myalgic encephalomyelitis. Journal of Infectious Diseases, 197 (8), 1171-1184. (doi:10.1086/533453). (PMID:18462164)

Record type: Article

Abstract

Chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is a multisystem disease, the pathogenesis of which remains undetermined. We set out to determine the precise abnormalities of gene expression in the blood of patients with CFS/ME. We analyzed gene expression in peripheral blood from 25 patients with CFS/ME diagnosed according to the Centers for Disease Control and Prevention diagnostic criteria and 50 healthy blood donors, using a microarray with a cutoff fold difference of expression of >or=2.5. Genes showing differential expression were further analyzed in 55 patients with CFS/ME and 75 healthy blood donors, using quantitative polymerase chain reaction. Differential expression was confirmed for 88 genes; 85 were upregulated, and 3 were downregulated. Highly represented functions were hematological disease and function, immunological disease and function, cancer, cell death, immune response, and infection. Clustering of quantitative polymerase chain reaction data from patients with CFS/ME revealed 7 subtypes with distinct differences in Medical Outcomes Survey Short Form-36 scores, clinical phenotypes, and severity.

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More information

e-pub ahead of print date: 19 March 2008
Published date: 15 April 2008
Organisations: Infection Inflammation & Immunity

Identifiers

Local EPrints ID: 70844
URI: http://eprints.soton.ac.uk/id/eprint/70844
ISSN: 0022-1899
PURE UUID: 0fd43c09-06ee-4825-acac-9a4a4d10cded

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Date deposited: 09 Mar 2010
Last modified: 13 Mar 2024 20:07

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Contributors

Author: Jonathan R. Kerr
Author: Robert Petty
Author: Beverley Burke
Author: John Gough
Author: David Fear
Author: Lindsey I. Sinclair
Author: Derek L. Mattey
Author: Selwyn C.M. Richards
Author: Jane Montgomery
Author: Don A. Baldwin
Author: Paul Kellam
Author: Tim J. Harrison
Author: George E. Griffin
Author: Janice Main
Author: Derek Enlander
Author: David J. Nutt

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