World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels
Humphreys, Tim, Andersson, Johan, Sodervall, Ulf and Melvin, Tracy (2009) World-to-chip interconnects for efficient loading of genomic DNA into microfluidic channels. Journal of Micromechanics and Microengineering, 19, (10), 105024-105032. (doi:10.1088/0960-1317/19/10/105024).
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Description/Abstract
A novel sloped interconnect for the efficient delivery of long genomic DNA fragments into a microfluidic channel is designed, fabricated and tested. Out-of-plane slopes are fabricated in silicon wafers using the deep reactive-ion etch lag phenomenon and a combination of anisotropic and isotropic etching. The final structure is capped with anodically bonded glass. Based upon a series of etch-lag calibration studies, the interconnect was designed using finite element analysis to provide a channel with flow acceleration properties appropriate to straighten DNA molecules. The efficiency of transit of the 48.5 kb DNA fragments (~16.5 µm long when fully extended) through the microfluidic device, established using quantitative real-time polymerase chain reaction, is 95 ± 7.3%
| Item Type: | Article |
|---|---|
| ISSNs: | 0960-1317 (print) |
| Related URLs: | |
| Subjects: | T Technology > TK Electrical engineering. Electronics Nuclear engineering Q Science > QC Physics |
| Divisions: | University Structure - Pre August 2011 > School of Electronics and Computer Science University Structure - Pre August 2011 > Optoelectronics Research Centre |
| Item ID: | 70952 |
| Date Deposited: | 11 Mar 2010 |
| Last Modified: | 01 Jun 2011 00:54 |
| Contributors: | Humphreys, Tim (Author) Andersson, Johan (Author) Sodervall, Ulf (Author) Melvin, Tracy (Author) |
| Date: | October 2009 |
| Status: | Published |
| Contact Email Address: | tm@orc.soton.ac.uk |
| URI: | http://eprints.soton.ac.uk/id/eprint/70952 |
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