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Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia

Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia
Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia
The serological analysis of recombinant cDNA expression libraries (SEREX) technique was used to immunoscreen a testes cDNA expression library with sera from newly diagnosed acute myeloid leukaemia (AML) patients. We used a testis cDNA library to aid our identification of cancer-testis (CT) antigens. We identified 44 antigens which we further immunoscreened with sera from AML, chronic myeloid leukaemia (CML), and normal donors. Eight antigens were solely recognised by patient sera including the recently described CT antigen, PASD1, and the cancer-related SSX2 interacting protein, SSX2IP. RT-PCR analysis indicated that we had identified three antigens which were expressed in patient bone marrow (BM) and peripheral blood (PB) but not in normal donor samples (PASD1, SSX2IP, and GRINL1A). Real-time PCR (RQ-PCR) confirmed the restricted expression of PASD1 in normal donor organs. Antigen presentation assays using monocyte-derived dendritic cells (mo-DCs) showed that PASD1 could stimulate autologous T-cell responses, suggesting that PASD1 could be a promising target for future immunotherapy clinical trials
immunotherapy, SEREX, acute myeloid leukaemia, cancer-testis antigens, PASD1, SSX2IP, chronic myeloid leukaemia, diffuse large B-cell lymphoma, leukaemia associated antigens, monocyte-derived dendritic cells
0006-291X
1293-1304
Guinn, Barbar-ann
596441de-9938-4994-9e04-0075cc32d205
Bland, Elizabeth A.
52ec125d-ecab-4efa-9fad-177d5430bcff
Lodi, Usman
58909343-2a56-4875-8714-076bbc0d5639
Liggins, Amanda P.
fe3f5c4b-ef41-4f09-b9fd-0dd602148d93
Tobal, Khalid
12607854-8ed3-4469-b18f-71db39547676
Petters, Sarah
66cba720-032c-4d3b-8421-c288946278ca
Wells, James W.
97a0a070-701b-46b0-81ba-9725595eb4e8
Banham, Alison H.
5be46579-6d51-42a8-a765-3cb92069e979
Mufti, Ghulam J.
940de420-bc41-4006-8517-f2c926ba70aa
Guinn, Barbar-ann
596441de-9938-4994-9e04-0075cc32d205
Bland, Elizabeth A.
52ec125d-ecab-4efa-9fad-177d5430bcff
Lodi, Usman
58909343-2a56-4875-8714-076bbc0d5639
Liggins, Amanda P.
fe3f5c4b-ef41-4f09-b9fd-0dd602148d93
Tobal, Khalid
12607854-8ed3-4469-b18f-71db39547676
Petters, Sarah
66cba720-032c-4d3b-8421-c288946278ca
Wells, James W.
97a0a070-701b-46b0-81ba-9725595eb4e8
Banham, Alison H.
5be46579-6d51-42a8-a765-3cb92069e979
Mufti, Ghulam J.
940de420-bc41-4006-8517-f2c926ba70aa

Guinn, Barbar-ann, Bland, Elizabeth A., Lodi, Usman, Liggins, Amanda P., Tobal, Khalid, Petters, Sarah, Wells, James W., Banham, Alison H. and Mufti, Ghulam J. (2005) Humoral detection of leukaemia-associated antigens in presentation acute myeloid leukaemia. Biochemical and Biophysical Research Communications, 335 (4), 1293-1304. (doi:10.1016/j.bbrc.2005.08.024).

Record type: Article

Abstract

The serological analysis of recombinant cDNA expression libraries (SEREX) technique was used to immunoscreen a testes cDNA expression library with sera from newly diagnosed acute myeloid leukaemia (AML) patients. We used a testis cDNA library to aid our identification of cancer-testis (CT) antigens. We identified 44 antigens which we further immunoscreened with sera from AML, chronic myeloid leukaemia (CML), and normal donors. Eight antigens were solely recognised by patient sera including the recently described CT antigen, PASD1, and the cancer-related SSX2 interacting protein, SSX2IP. RT-PCR analysis indicated that we had identified three antigens which were expressed in patient bone marrow (BM) and peripheral blood (PB) but not in normal donor samples (PASD1, SSX2IP, and GRINL1A). Real-time PCR (RQ-PCR) confirmed the restricted expression of PASD1 in normal donor organs. Antigen presentation assays using monocyte-derived dendritic cells (mo-DCs) showed that PASD1 could stimulate autologous T-cell responses, suggesting that PASD1 could be a promising target for future immunotherapy clinical trials

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More information

Published date: 7 October 2005
Keywords: immunotherapy, SEREX, acute myeloid leukaemia, cancer-testis antigens, PASD1, SSX2IP, chronic myeloid leukaemia, diffuse large B-cell lymphoma, leukaemia associated antigens, monocyte-derived dendritic cells

Identifiers

Local EPrints ID: 71573
URI: http://eprints.soton.ac.uk/id/eprint/71573
ISSN: 0006-291X
PURE UUID: 258d6772-80c4-4b3e-aa1b-1b79c2f5b7a9

Catalogue record

Date deposited: 14 Dec 2009
Last modified: 13 Mar 2024 20:33

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Contributors

Author: Barbar-ann Guinn
Author: Elizabeth A. Bland
Author: Usman Lodi
Author: Amanda P. Liggins
Author: Khalid Tobal
Author: Sarah Petters
Author: James W. Wells
Author: Alison H. Banham
Author: Ghulam J. Mufti

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