Applying full-field measurement method to Pseudomonas aeruginosa biofilms

Mathias, J.D. and Stoodley, P. (2009) Applying full-field measurement method to Pseudomonas aeruginosa biofilms. Biofouling, 25, (8), 695-703. (doi:10.1080/08927010903104984).


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A digital image correlation (DIC) method was applied to characterize the mechanical behavior of Pseudomonas aeruginosa biofilms in response to wall shear stress using digital video micrographs taken from biofilm flow cells. The appearance of the biofilm in the transmitted light photomicrographs presented a natural texture which was highly conducive to random encoding for DIC. The displacement fields were calculated for two biofilm specimens. The DIC method concurred with previous analysis showing that biofilms exhibit viscoelastic behavior, but had the advantage over simple length measurements of longitudinal strain that it could precisely measure local strains in length (x) and width (y) within biofilm clusters with a 2 ?m resolution as a function of time and wall shear stress. It was concluded that DIC was more accurate at measuring elastic moduli than simple length measurements, but that time-lapse 3D images would enable even more accurate estimates to be performed

Item Type: Article
Digital Object Identifier (DOI): doi:10.1080/08927010903104984
Additional Information: The National Centre for Advanced Tribology at Southampton (nCATS)
ISSNs: 0892-7014 (print)
Related URLs:
Keywords: bacterial biofilm, digital image correlation, mechanical response
Subjects: T Technology > T Technology (General)
Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Engineering Sciences
ePrint ID: 71653
Accepted Date and Publication Date:
November 2009Published
Date Deposited: 18 Dec 2009
Last Modified: 31 Mar 2016 13:03

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