Biofilm formation by ica positive and ica negative strains of Staphylococcus epidermidis in vitro
Biofilm formation by ica positive and ica negative strains of Staphylococcus epidermidis in vitro
Staphylococcus epidermidis is a clinically important opportunistic pathogen that forms biofilm infections on nearly all types of indwelling medical devices. The biofilm forming capability of S. epidermidis has been linked to the presence of the ica operon in the genome, and the amount of biofilm formation measured by the crystal violet (CV) adherence assay. Six S. epidermidis strains were characterized for their ica status using PCR, and their biofilm forming ability over 6 days, using the CV assay and a flow cell system. Ica-negative strains characterized as 'negative for biofilm formation' based on the CV assay were demonstrated to form strongly attached biofilms after 6 days. However, the biofilms were not as extensive as the ica-positive strains. It was concluded that ica is not required for biofilm formation, nor is the 24-h CV assay generalizable for predicting the 6-day biofilm-forming ability for all S. epidermidis strains
biofilm, ica, staphylococcus epidermidis, biofilm forming index, flow cells
367-375
Dice, Bethany
72120cd5-bde7-4799-bba8-38cc906d14b1
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Buchinsky, Farrel
dc3f9411-86ba-4f6f-a841-9b55cd64d6b3
Metha, Nalini
c51f30fd-8067-4f29-a29e-2e7b8ccf82ce
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Hu, Fen Z.
0073d77a-082f-4652-b467-0ba1c709fc94
May 2009
Dice, Bethany
72120cd5-bde7-4799-bba8-38cc906d14b1
Stoodley, P.
08614665-92a9-4466-806e-20c6daeb483f
Buchinsky, Farrel
dc3f9411-86ba-4f6f-a841-9b55cd64d6b3
Metha, Nalini
c51f30fd-8067-4f29-a29e-2e7b8ccf82ce
Ehrlich, Garth D.
aa8e5162-77a6-4627-a793-acd724ed0782
Hu, Fen Z.
0073d77a-082f-4652-b467-0ba1c709fc94
Dice, Bethany, Stoodley, P., Buchinsky, Farrel, Metha, Nalini, Ehrlich, Garth D. and Hu, Fen Z.
(2009)
Biofilm formation by ica positive and ica negative strains of Staphylococcus epidermidis in vitro.
Biofouling, 4 (25), .
(doi:10.1080/08927010902803297).
Abstract
Staphylococcus epidermidis is a clinically important opportunistic pathogen that forms biofilm infections on nearly all types of indwelling medical devices. The biofilm forming capability of S. epidermidis has been linked to the presence of the ica operon in the genome, and the amount of biofilm formation measured by the crystal violet (CV) adherence assay. Six S. epidermidis strains were characterized for their ica status using PCR, and their biofilm forming ability over 6 days, using the CV assay and a flow cell system. Ica-negative strains characterized as 'negative for biofilm formation' based on the CV assay were demonstrated to form strongly attached biofilms after 6 days. However, the biofilms were not as extensive as the ica-positive strains. It was concluded that ica is not required for biofilm formation, nor is the 24-h CV assay generalizable for predicting the 6-day biofilm-forming ability for all S. epidermidis strains
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Published date: May 2009
Additional Information:
The National Centre for Advanced Tribology at Southampton (nCATS)
Keywords:
biofilm, ica, staphylococcus epidermidis, biofilm forming index, flow cells
Identifiers
Local EPrints ID: 71659
URI: http://eprints.soton.ac.uk/id/eprint/71659
ISSN: 0892-7014
PURE UUID: 9f93dae4-e665-4ece-95e5-20ed5940ffdc
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Date deposited: 17 Dec 2009
Last modified: 14 Mar 2024 02:55
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Contributors
Author:
Bethany Dice
Author:
Farrel Buchinsky
Author:
Nalini Metha
Author:
Garth D. Ehrlich
Author:
Fen Z. Hu
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