Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges
Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.
1029-1042
Wiedenmann, Joerg
ad445af2-680f-4927-90b3-589ac9d538f7
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Nienhaus, Gerd Ulrich
51f382ec-d401-4cbb-a4b2-8e06045fc212
2009
Wiedenmann, Joerg
ad445af2-680f-4927-90b3-589ac9d538f7
Oswald, Franz
a5b02f2d-8439-411b-b5ad-999629cee58f
Nienhaus, Gerd Ulrich
51f382ec-d401-4cbb-a4b2-8e06045fc212
Wiedenmann, Joerg, Oswald, Franz and Nienhaus, Gerd Ulrich
(2009)
Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges.
IUMBM Life, 61 (11), .
(doi:10.1002/iub.256).
Abstract
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.
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Published date: 2009
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Local EPrints ID: 71720
URI: http://eprints.soton.ac.uk/id/eprint/71720
ISSN: 1521-6543
PURE UUID: 9b54f1ee-66f9-4834-8a3d-a827946ef943
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Date deposited: 18 Dec 2009
Last modified: 14 Mar 2024 02:52
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Author:
Franz Oswald
Author:
Gerd Ulrich Nienhaus
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