Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Wiedenmann, Joerg, Oswald, Franz and Nienhaus, Gerd Ulrich (2009) Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges. IUMBM Life, 61, (11), 1029-1042. (doi:10.1002/iub.256).


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The green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1002/iub.256
ISSNs: 1521-6543 (print)
Related URLs:
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions : University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES)
ePrint ID: 71720
Accepted Date and Publication Date:
Date Deposited: 18 Dec 2009
Last Modified: 31 Mar 2016 13:03
URI: http://eprints.soton.ac.uk/id/eprint/71720

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