Implication of nitric oxide in the heat-stress-induced cell death of the symbiotic alga Symbiodinium microadriaticum
Bouchard, Josée Nina and Yamasaki, Hideo (2009) Implication of nitric oxide in the heat-stress-induced cell death of the symbiotic alga Symbiodinium microadriaticum. Marine Biology, 156, (11), 2209-2220. (doi:10.1007/s00227-009-1249-3).
Download
Full text not available from this repository.
Description/Abstract
One of the major consequences of global warming is a rise in sea surface temperature which may affect the survival of marine organisms including phytoplankton. Here, we provide experimental evidence for heat-induced cell death in a symbiotic microalga. Shifting Symbiodinium microadriaticum from 27 to 32°C resulted in an increase in mortality, an increase in caspase 3-like activity, and an increase in nitric oxide (NO) production. The caspase-like activity was strongly correlated with the production of NO in thermally challenged microalgae. For this experiment, the application of Ac-DEVD-CHO, a mammalian caspase 3-specific inhibitor, partly prevented (by 65%) the increase in caspase-like activity. To verify the relationship between NO and the caspase-like activity, S. microadriaticum were subsequently incubated with 1.0 mM of the following chemical NO donors: sodium nitroprusside (SNP), S-nitrosoglutathione (GSNO), S-nitroso-N-acetylpenicillamine (SNAP) and 3,3bis(Aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18). The supplementation of both SNP and NOC-18 caused a significant increase in caspase-like activity compared to the control treatment. Pre-treatment of the microalgae with the inhibitor Ac-DEVD-CHO before the supplementation of the different NO donors completely prevented the increase in caspase-like activity. These results suggest that NO could play a role in the induction of cell death in heat-stressed S. microadriaticum by mediating an increase in caspase-like activity.
| Item Type: | Article |
|---|---|
| ISSNs: | 0025-3162 (print) |
| Related URLs: | |
| Subjects: | Q Science > QH Natural history > QH301 Biology G Geography. Anthropology. Recreation > GC Oceanography |
| Divisions: | University Structure - Pre August 2011 > School of Ocean & Earth Science (SOC/SOES) |
| Item ID: | 71763 |
| Date Deposited: | 22 Dec 2009 |
| Last Modified: | 01 Jun 2011 15:08 |
| Contributors: | Bouchard, Josée Nina (Author) Yamasaki, Hideo (Author) |
| Date: | October 2009 |
| Status: | Published |
| URI: | http://eprints.soton.ac.uk/id/eprint/71763 |
Actions (login required)
 |
View Item |
