Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer
Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer
Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustness can be achieved if integrated optics are used instead of traditional free space optics. We present designs simulation and experimental characterisation of integrated optics for a microfabricated cytometer made from SU-8 resin on a glass substrate. The optics constructed from combinations of optical fibres (positioned with microgrooves), waveguides, and microlenses enable analysis of scattered light and fluorescence from particles positioned near the centre of a microchannel using one dimensional sheath flow. Four different methods for directing the incident light onto the particles are examined and the optimum design discussed.
1987-1992
Barat, David
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Benazzi, Guiseppe
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Mowlem, Matthew Charles
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Ruano, Jesus Miguel
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Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
1 May 2010
Barat, David
c7bf457e-3aef-48af-85ed-dfcee368ca76
Benazzi, Guiseppe
0a1faa41-fbc8-426b-a942-f35d44757ebd
Mowlem, Matthew Charles
6f633ca2-298f-48ee-a025-ce52dd62124f
Ruano, Jesus Miguel
a3c383c1-907e-440d-9e42-bd1bf0cd4097
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Barat, David, Benazzi, Guiseppe, Mowlem, Matthew Charles, Ruano, Jesus Miguel and Morgan, Hywel
(2010)
Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer.
Optics Communications, 283 (9), .
(doi:10.1016/j.optcom.2009.12.046).
Abstract
Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustness can be achieved if integrated optics are used instead of traditional free space optics. We present designs simulation and experimental characterisation of integrated optics for a microfabricated cytometer made from SU-8 resin on a glass substrate. The optics constructed from combinations of optical fibres (positioned with microgrooves), waveguides, and microlenses enable analysis of scattered light and fluorescence from particles positioned near the centre of a microchannel using one dimensional sheath flow. Four different methods for directing the incident light onto the particles are examined and the optimum design discussed.
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Published date: 1 May 2010
Organisations:
Ocean Technology and Engineering
Identifiers
Local EPrints ID: 72160
URI: http://eprints.soton.ac.uk/id/eprint/72160
ISSN: 0030-4018
PURE UUID: ec6d718a-b93a-4c9b-8c3f-7b1c181131f2
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Date deposited: 26 Jan 2010
Last modified: 14 Mar 2024 02:48
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Contributors
Author:
David Barat
Author:
Guiseppe Benazzi
Author:
Matthew Charles Mowlem
Author:
Jesus Miguel Ruano
Author:
Hywel Morgan
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