Tissue specific genetic regulation of
Interleukin 6
Tissue specific genetic regulation of
Interleukin 6
Interleukin 6 (IL6) is associated with arterial disease development, progression and
surgical outcome. Raised levels of IL6 may play a causal role in disease development
or may be the effect of pathology. An IL6 single nucleotide polymorphism (SNP) G-
174C has been identified and reported to associate with IL6 expression. However,
conflicting results have emerged and both the relationship between IL6 and vascular
disease and the precise effect of SNP G-174C in vivo in humans remains obscure.
The aim of this study was to establish the effect of SNP G-174C in humans, in vivo
in different tissues. Varicose vein surgery patients donated adipose tissue, skeletal
muscle, vein and blood samples. Patients were genotyped for SNP G-174C. A new
pre-mRNA assay was developed, using gel electrophoresis, restriction digest and
fluorescence quantification, to measure the ratio of heterozygous allelic pre-mRNA
transcription. IL6 mRNA expression in different tissues was also measured using
relative real time PCR (RT-PCR) to assess effect of tissue type on expression profiles.
mRNA expression within tissues was compared between G-174C genotypes, to
further quantifying the association of SNP G-174C with transcript levels.
The pre-mRNA assay showed higher expression for the C-allele, though not
statistically significant. The pre-mRNA assay needed to detect low levels of intron
retaining allelic pre-mRNA isoforms. Replicates and controls for residual genomic
DNA were used to monitor assay precision. Adipose tissue gave the greatest
precision in the pre-mRNA assay. In the RT PCR assay adipose tissue expressed
significantly more IL6 mRNA than all other tissues examined. In vein and leukocytes
subjects with the CC genotype expressed significantly higher levels of IL6 mRNA
than subjects with GC or GG genotypes. There was a trend towards higher
expression for the CC genotype in all tissue types. A significant though weak
correlation between IL6 mRNA expression and age was demonstrated for vein and
leukocytes.
Adipose tissue may be an important source of IL6 compared to other tissues. This
may be relevant for obesity associated diseases. Subjects with G-174C genotype CC
showed a trend towards higher IL6 RNA expression. Further studies are necessary to
clarify the effect of genotype on IL6 expression.
Sonnenberg, Sabine
c864b953-04f5-43dc-9dd5-1d254a654619
July 2009
Sonnenberg, Sabine
c864b953-04f5-43dc-9dd5-1d254a654619
Shearman, C.
cf4d6317-f54d-4ab3-ba49-c6797897bbcf
Day, I.
5f36cc51-57f8-4eef-9ebb-d91f0c53f295
Sonnenberg, Sabine
(2009)
Tissue specific genetic regulation of
Interleukin 6.
University of Southampton, School of Medicine, Doctoral Thesis, 283pp.
Record type:
Thesis
(Doctoral)
Abstract
Interleukin 6 (IL6) is associated with arterial disease development, progression and
surgical outcome. Raised levels of IL6 may play a causal role in disease development
or may be the effect of pathology. An IL6 single nucleotide polymorphism (SNP) G-
174C has been identified and reported to associate with IL6 expression. However,
conflicting results have emerged and both the relationship between IL6 and vascular
disease and the precise effect of SNP G-174C in vivo in humans remains obscure.
The aim of this study was to establish the effect of SNP G-174C in humans, in vivo
in different tissues. Varicose vein surgery patients donated adipose tissue, skeletal
muscle, vein and blood samples. Patients were genotyped for SNP G-174C. A new
pre-mRNA assay was developed, using gel electrophoresis, restriction digest and
fluorescence quantification, to measure the ratio of heterozygous allelic pre-mRNA
transcription. IL6 mRNA expression in different tissues was also measured using
relative real time PCR (RT-PCR) to assess effect of tissue type on expression profiles.
mRNA expression within tissues was compared between G-174C genotypes, to
further quantifying the association of SNP G-174C with transcript levels.
The pre-mRNA assay showed higher expression for the C-allele, though not
statistically significant. The pre-mRNA assay needed to detect low levels of intron
retaining allelic pre-mRNA isoforms. Replicates and controls for residual genomic
DNA were used to monitor assay precision. Adipose tissue gave the greatest
precision in the pre-mRNA assay. In the RT PCR assay adipose tissue expressed
significantly more IL6 mRNA than all other tissues examined. In vein and leukocytes
subjects with the CC genotype expressed significantly higher levels of IL6 mRNA
than subjects with GC or GG genotypes. There was a trend towards higher
expression for the CC genotype in all tissue types. A significant though weak
correlation between IL6 mRNA expression and age was demonstrated for vein and
leukocytes.
Adipose tissue may be an important source of IL6 compared to other tissues. This
may be relevant for obesity associated diseases. Subjects with G-174C genotype CC
showed a trend towards higher IL6 RNA expression. Further studies are necessary to
clarify the effect of genotype on IL6 expression.
Text
Thesis_43_corrected_after_viva_july09_submitted.pdf
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More information
Published date: July 2009
Organisations:
University of Southampton
Identifiers
Local EPrints ID: 72590
URI: http://eprints.soton.ac.uk/id/eprint/72590
PURE UUID: aa5dbf06-6bd0-4681-93e0-3ab52d802c1c
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Date deposited: 17 Feb 2010
Last modified: 13 Mar 2024 21:35
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Contributors
Author:
Sabine Sonnenberg
Thesis advisor:
I. Day
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