Stimulation of lactate production in human granulosa cells by metformin and potential involvement of adenosine 5' monophosphate-activated protein kinase

Richardson, Malcolm C., Ingamells, Susan, Simonis, Chantal D., Cameron, Iain T., Sreekumar, Rajiv, Vijendren, Ananth, Sellahewa, Luckni, Coakley, Stephanie and Byrne, Christopher D. (2009) Stimulation of lactate production in human granulosa cells by metformin and potential involvement of adenosine 5' monophosphate-activated protein kinase. Journal of Clinical Endocrinology & Metabolism, 94, (2), 670-677. (doi:10.1210/jc.2008-2025). (PMID:19001513).


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Context: production of 3-carbon units (as lactate) by granulosa cells (GCs) is important in follicular and oocyte development and may be modulated by metformin.

Objective: the aim of the study was to examine the action of metformin on GC lactate production and potential mediation via AMP-activated protein kinase (AMPK).

Design: GCs were prepared from follicular aspirates. After exposure to metformin and other potential modulators of AMPK in culture, aspects of cellular function were examined.

Setting: the study was conducted in a private fertility clinic/university academic center.

Patients: women undergoing routine in vitro fertilization participated in the study.

Interventions: all agents were added in culture.

Main outcome measures: lactate output of GCs was measured. Cell extracts were prepared after culture, and phosphorylated forms of AMPK and acetyl CoA carboxylase (ACC) were assayed using Western analysis.

Results: metformin led to a rapid increase in lactate production by GCs [minimum effective dose, 250 mu M; maximum dose studied, 1 mM (1.22-fold; P < 0.01)]. This dose range of metformin was similar to that required for stimulation of phospho-AMPK in GCs [minimum effective dose, 250 mu M; maximum effect, 500 mu M (2.01-fold; P < 0.001)]. Increasing phospho-ACC, as a representative downstream target regulated by AMPK, was apparent over a lower range (minimum effective dose, 31 mu M; maximum effect, 250 mu M; P < 0.001). A level of metformin (125 mu M) insufficient for the stimulation of lactate output when used alone potentiated the effects of suboptimal doses of insulin on lactate production. Adiponectin (2.5 mu g/ml) had a small but significant effect on lactate output.

Conclusions: metformin activates AMPK in GCs, stimulating lactate production and increasing phospho-ACC. Metformin also enhances the action of suboptimal insulin concentrations to stimulate lactate production. (J Clin Endocrinol Metab 94: 670-677, 2009

Item Type: Article
Digital Object Identifier (DOI): doi:10.1210/jc.2008-2025
ISSNs: 0021-972X (print)
Subjects: Q Science > QP Physiology
R Medicine > RB Pathology
R Medicine > RC Internal medicine
Divisions : University Structure - Pre August 2011 > School of Medicine > Developmental Origins of Health and Disease
ePrint ID: 72698
Accepted Date and Publication Date:
February 2009Published
Date Deposited: 22 Feb 2010
Last Modified: 31 Mar 2016 13:04

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